Structural Characterization of Thiaminase type II TenA from Staphylococcus aureus

MEFSQKLYQAAKPIINDIYEDDFIQKMLLGNIQADALRHYLQADAAYLKEFTNLYALLIPKMNSMNDVKFLVEQIEFMVE
GEVLAHDILAQIVGESYEEIIKTKVWPPSGDHYIKHMYFQAHSRENAIYTIAAMAP(CME)PYIYAELAKRSQSDHKLNR
EKDTAKWFDFYSTEMDDIINVFESLMNKLAESMSDKELEQVKQVFLESCIHERRFFNMAMTLEQWEFGGKVND

MEFSQKLYQAAKPIINDIYEDDFIQKMLLGNIQADALRHYLQADAAYLKEFTNLYALLIPKMNSMNDVKFLVEQIEFMVE
GEVLAHDILAQIVGESYEEIIKTKVWPPSGDHYIKHMYFQAHSRENAIYTIAAMAPCPYIYAELAKRSQSDHKLNREKDT
AKWFDFYSTEMDDIINVFESLMNKLAESMSDKELEQVKQVFLESCIHERRFFNMAMTLEQWEFGGKVND

28%(w/v) PEG 3500, 0.2 M sodium acetate and 0.1 M Tris HCl buffer pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

alpha-helix, thiaminase, Vitamin B1, bi-functional enzyme, cleavage of thiamine into HMP and THZ, deamination of amino-pyrimidine to HMP in S.aureus, HYDROLASE, TRANSCRIPTION