Crystal Structure Analysis of a GH30 Endoxylanase from Clostridium papyrosolvens C71

A novel member of glycoside hydrolase family 30 subfamily 8 with altered substrate specificity.

Glucuronoarabinoxylan endo-1,4-beta-xylanase

MASDVTVNLGSTKQEIRGFGASSAWCGTISDYVMNSLYGDLGYSILRLRIEEGIGDAWKTGNFSKWSPELANAKKASAKG
AIVFASPWNPPASMQENFSKSGDSSAQRLRYDKYTEYAQYLNAYVKYMKDNGVDLYAISVQNEPDYAQDWTWWTPQEMLN
FMKNNAGSINCRVMAPESFQFLKNMSDPILNDATALDNMDVLGCHFYGTSVNNMAYPLYQQKSAGKELWMTEKYFDDDTT
GNIMNMSKEIHDSMVTGNMNAYIYWWITWPNGLATSSGTIYKRAYVLGQFAKFIRPGYKRVDATATPNTNVYVSAYTGDN
KAVIVAINTGTAAVSQKFNFQNGSASSVVSYVTDSSRNMAAGANIAVTNGSFTAQLPAQSITTFVGNALEHHHHHH

0.1M Ammonium acetate, 0.1M BIS-TRIS, 17% PEG10,000, pH pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K

ALPHA BETA BARREL, (BETA/ALPHA)8 BARREL, HYDROLASE

ASDVTVNLGSTKQEIRGFGASSAWCGTISDYVMNSLYGDLGYSILRLRIEEGIGDAWKTGNFSKWSPELANAKKASAKGA
IVFASPWNPPASMQENFSKSGDSSAQRLRYDKYTEYAQYLNAYVKYMKDNGVDLYAISVQNEPDYAQDWTWWTPQEMLNF
MKNNAGSINCRVMAPESFQFLKNMSDPILNDATALDNMDVLGCHFYGTSVNNMAYPLYQQKSAGKELWMTEKYFDDDTTG
NIMNMSKEIHDSMVTGNMNAYIYWWITWPNGLATSSGTIYKRAYVLGQFAKFIRPGYKRVDATATPNTNVYVSAYTGDNK
AVIVAINTGTAAVSQKFNFQNGSASSVVSYVTDSSRNMAAGANIAVTNGSFTAQLPAQSITTFVGN