Structure of C126S mutant of Saccharomyces cerevisiae triosephosphate isomerase

Effects of a buried cysteine-to-serine mutation on yeast triosephosphate isomerase structure and stability.

MARTFFVGGNFKLNGSKQSIKEIVERLNTASIPENVEVVICPPATYLDYSVSLVKKPQVTVGAQNAYLKASGAFTGENSV
DQIKDVGAKWVILGHSERRSYFHEDDKFIADKTKFALGQGVGVILSIGETLEEKKAGKTLDVVERQLNAVLEEVKDWTNV
VVAYEPVWAIGTGLAATPEDAQDIHASIRKFLASKLGDKAASELRILYGGSANGSNAVTFKDKADVDGFLVGGASLKPEF
VDIINSRN

Ammonium sulfate 2.2 M, 0.01 M cobaltous chloride hexahydrate in 0.01 M MES, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 291K

(ALFA/BETA )8 barrel, ISOMERASE, OXIDOREDUCTASE

MARTFFVGGNFKLNGSKQSIKEIVERLNTASIPENVEVVICPPATYLDYSVSLVKKPQVTVGAQNAYLKASGAFTGENSV
DQIKDVGAKWVILGHSERRSYFHEDDKFIADKTKFALGQGVGVILCIGETLEEKKAGKTLDVVERQLNAVLEEVKDWTNV
VVAYEPVWAIGTGLAATPEDAQDIHASIRKFLASKLGDKAASELRILYGGSANGSNAVTFKDKADVDGFLVGGASLKPEF
VDIINSRN