Crystal structure of an unfused Mre11-Nbs1 complex with two manganese ions per active site

Structure of Mre11-Nbs1 complex yields insights into ataxia-telangiectasia-like disease mutations and DNA damage signaling.

DNA repair and telomere maintenance protein nbs1

GPDMNNELHNENTIRILISSDPHVGYGEKDPVRGNDSFVSFNEILEIARERDVDMILLGGDIFHDNKPSRKALYQALRSL
RLNCLGDKPCELELLSDTSLTTGDTAVCNINYLDPNINVAIPVFSIHGNHDDPSGDGRYSALDILQVTGLVNYFGRVPEN
DNIVVSPILLQKGFTKLALYGISNVRDERLYHSFRENKVKFLRPDLYRDEWFNLLTVHQNHSAHTPTSYLPESFIQDFYD
FVLWGHEHECLIDGSYNPTQKFTVVQPGSTIATSLSPGETAPKHCGILNITGKDFHLEKIRLRTVRPFIMKDIILSEVSS
IPPMVENKKEVLTYLISKVEEAITEANAQWYEAQGTVPVVENEKPPLPLIRLRVDYTGGYQTENPQRFSNRFVGRVANAT
DVVQFYLKKVDENLYFQ

200 mM ammonium citrate pH 5.5 14 % PEG 3350 50 mM MnCl2, VAPOR DIFFUSION, HANGING DROP

DNA double-strand break repair, Nuclease, Hydrolase, HYDROLASE-PROTEIN BINDING complex

DMNNELHNENTIRILISSDPHVGYGEKDPVRGNDSFVSFNEILEIARERDVDMILLGGDIFHDNKPSRKALYQALRSLRL
NCLGDKPCELELLSDTSLTTGDTAVCNINYLDPNINVAIPVFSIHGNHDDPSGDGRYSALDILQVTGLVNYFGRVPENDN
IVVSPILLQKGFTKLALYGISNVRDERLYHSFRENKVKFLRPDLYRDEWFNLLTVHQNHSAHTPTSYLPESFIQDFYDFV
LWGHEHECLIDGSYNPTQKFTVVQPGSTIATSLSPGETAPKHCGILNITGKDFHLEKIRLRTVRPFIMKDIILSEVSSIP
PMVENKKEVLTYLISKVEEAITEANAQWYEAQGTVPVVENEKPPLPLIRLRVDYTGGYQTENPQRFSNRFVGRVANATDV
VQFYLKK