X-ray structure of WDR5-MLL1 Win motif peptide binary complex

Structural basis for WDR5 interaction (Win) motif recognition in human SET1 family histone methyltransferases.

ALL-1, CXXC-type zinc finger protein 7, Lysine N-methyltransferase 2A, KMT2A, Trithorax-like protein, Zinc finger protein HRX, MLL cleavage product N320, N-terminal cleavage product of 320 kDa, p320, MLL cleavage product C180, C-terminal cleavage product of 180 kDa, p180

ATQSKPTPVKPNYALKFTLAGHTKAVSSVKFSPNGEWLASSSADKLIKIWGAYDGKFEKTISGHKLGISDVAWSSDSNLL
VSASDDKTLKIWDVSSGKCLKTLKGHSNYVFCCNFNPQSNLIVSGSFDESVRIWDVKTGKCLKTLPAHSDPVSAVHFNRD
GSLIVSSSYDGLCRIWDTASGQCLKTLIDDDNPPVSFVKFSPNGKYILAATLDNTLKLWDYSKGKCLKTYTGHKNEKYCI
FANFSVTGGKWIVSGSEDNLVYIWNLQTKEIVQKLQGHTDVVISTACHPTENIIASAALENDKTIKLWKSDC

PEG3350, Ammonium Sulfate, HEPES, pH 7.2, hanging drop, temperature 294K

WD40, Win motif, beta propeller, 3-10 helix, lysine methyltransferase, RbBP5, MLL1, Ash2L, core complex, Histone, Transcription-Transferase complex