The crystal structure of the ferric yersiniabactin uptake receptor FyuA from Yersinia pestis

Structural engineering of a phage lysin that targets Gram-negative pathogens.

Ferric yersiniabactin uptake receptor, FyuA, IRPC

GA(MSE)GQTSQQDESTLVVTASKQSSRSASANNVSSTVVSAPELSDAGVTASDKLPRVLPGLNIENSGN(MSE)LFSTI
SLRGVSSAQDFYNPAVTLYVDGVPQLSTNTIQALTDVQSVELLRGPQGTLYGKSAQGGIINIVTQQPDSTPRGYIEGGVS
SRDSYRSKFNLSGPIQDGLLYGSVTLLRQVDDGD(MSE)INPATGSDDLGGTRASIGNVKLRLAPDDQPWE(MSE)GFAA
SRECTRATQDAYVGWNDIKGRKLSISDGSPDPY(MSE)RRCTDSQTLSGKYTTDDWVFNLISAWQQQHYSRTFPSGSLIV
N(MSE)PQRWNQDVQELRAATLGDARTVD(MSE)VFGLYRQNTREKLNSAYD(MSE)PT(MSE)PYLSSTGYTTAETLAA
YSDLTWHLTDRFDIGGGVRFSHDKSSTQYHGS(MSE)LGNPFGDQGKSNDDQVLGQLSAGY(MSE)LTDDWRVYTRVAQG
YKPSGYNIVPTAGLDAKPFVAEKSINYELGTRYETADVTLQAATFYTHTKD(MSE)QLYSGPVR(MSE)QTLSNAGKADA
TGVELEAKWRFAPGWSWDINGNVIRSEFTNDSELYHGNRVPFVPRYGAGSSVNGVIDTRYGAL(MSE)PRLAVNLVGPHY
FDGDNQLRQGTYATLDSSLGWQATER(MSE)NISVYVDNLFDRRYRTYGY(MSE)NGSSAVAQVN(MSE)GRTVGINTRI
DFF

GAMGQTSQQDESTLVVTASKQSSRSASANNVSSTVVSAPELSDAGVTASDKLPRVLPGLNIENSGNMLFSTISLRGVSSA
QDFYNPAVTLYVDGVPQLSTNTIQALTDVQSVELLRGPQGTLYGKSAQGGIINIVTQQPDSTPRGYIEGGVSSRDSYRSK
FNLSGPIQDGLLYGSVTLLRQVDDGDMINPATGSDDLGGTRASIGNVKLRLAPDDQPWEMGFAASRECTRATQDAYVGWN
DIKGRKLSISDGSPDPYMRRCTDSQTLSGKYTTDDWVFNLISAWQQQHYSRTFPSGSLIVNMPQRWNQDVQELRAATLGD
ARTVDMVFGLYRQNTREKLNSAYDMPTMPYLSSTGYTTAETLAAYSDLTWHLTDRFDIGGGVRFSHDKSSTQYHGSMLGN
PFGDQGKSNDDQVLGQLSAGYMLTDDWRVYTRVAQGYKPSGYNIVPTAGLDAKPFVAEKSINYELGTRYETADVTLQAAT
FYTHTKDMQLYSGPVRMQTLSNAGKADATGVELEAKWRFAPGWSWDINGNVIRSEFTNDSELYHGNRVPFVPRYGAGSSV
NGVIDTRYGALMPRLAVNLVGPHYFDGDNQLRQGTYATLDSSLGWQATERMNISVYVDNLFDRRYRTYGYMNGSSAVAQV
NMGRTVGINTRIDFF

2.4 M ammonium formate, 0.1 M HEPES, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

tonB dependent transporter, iron import, METAL TRANSPORT

QTSQQDESTLVVTASKQSSRSASANNVSSTVVSAPELSDAGVTASDKLPRVLPGLNIENSGNMLFSTISLRGVSSAQDFY
NPAVTLYVDGVPQLSTNTIQALTDVQSVELLRGPQGTLYGKSAQGGIINIVTQQPDSTPRGYIEGGVSSRDSYRSKFNLS
GPIQDGLLYGSVTLLRQVDDGDMINPATGSDDLGGTRASIGNVKLRLAPDDQPWEMGFAASRECTRATQDAYVGWNDIKG
RKLSISDGSPDPYMRRCTDSQTLSGKYTTDDWVFNLISAWQQQHYSRTFPSGSLIVNMPQRWNQDVQELRAATLGDARTV
DMVFGLYRQNTREKLNSAYDMPTMPYLSSTGYTTAETLAAYSDLTWHLTDRFDIGGGVRFSHDKSSTQYHGSMLGNPFGD
QGKSNDDQVLGQLSAGYMLTDDWRVYTRVAQGYKPSGYNIVPTAGLDAKPFVAEKSINYELGTRYETADVTLQAATFYTH
TKDMQLYSGPVRMQTLSNAGKADATGVELEAKWRFAPGWSWDINGNVIRSEFTNDSELYHGNRVPFVPRYGAGSSVNGVI
DTRYGALMPRLAVNLVGPHYFDGDNQLRQGTYATLDSSLGWQATERMNISVYVDNLFDRRYRTYGYMNGSSAVAQVNMGR
TVGINTRIDFF