Structural and Biochemical Characterization of the Vaccinia Virus Envelope Protein D8 and Its Recognition by the Antibody LA5.

Side scattering I-beam bent single crystal, asymmetric cut 4.9650 degrees

extracellular domain (UNP residues 1-261)

MPQQLSPINIETKKAISNARLKPLDIHYNESKPTTIQNTGKLVRINFKGGYISGGFLPNEYVLSSLHIYWGKEDDYGSNH
LIDVYKYSGEINLVHWNKKKYSSYEEAKKHDDGLIIISIFLQVLDHKNVYFQKIVNQLDSIRSANTSAPFDSVFYLDNLL
PSKLDYFTYLGTTINHSADAVWIIFPTPINIHSDQLSKFRTLLSLSNHEGKPHYITENYRNPYKLNDDTEVYYSGEIIRA
ATTSPARENYFMRWLSDLRETLEHHHHHH

0.2 M sodium iodide, 20% PEG3350, VAPOR DIFFUSION, temperature 298K

Crystal Structure of Vaccinia Virus L1 protein

Structure of Fab 7D11 from a neutralizing antibody against the poxvirus L1 protein

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : WITH TLS ADDED

CAH alpha fold, Vp7 motif, beta sheet, cell surface chondroitin binding, viral entry, chondroitin sulfate, VIRAL PROTEIN

MPQQLSPINIETKKAISNARLKPLDIHYNESKPTTIQNTGKLVRINFKGGYISGGFLPNEYVLSSLHIYWGKEDDYGSNH
LIDVYKYSGEINLVHWNKKKYSSYEEAKKHDDGLIIISIFLQVLDHKNVYFQKIVNQLDSIRSANTSAPFDSVFYLDNLL
PSKLDYFTYLGTTINHSADAVWIIFPTPINIHSDQLSKFRTLLSLSNHEGKPHYITENYRNPYKLNDDTEVYYSGEIIRA
ATTSPARENYFMRWLSDLRET