Crystal Structure of Nattokinase from Bacillus subtilis natto

Purification, crystallization and preliminary X-ray diffraction experiment of nattokinase from Bacillus subtilis natto

AQSVPYGISQIKAPALHSQGYTGSNVKVAVIDSGIDSSHPDLNVRGGASFVPSETNPYQDGSSHGTHVAGTIAALNNSIG
VLGVAPSASLYAVKVLDSTGSGQYSWIINGIEWAISNNMDVINMSLGGPTGSTALKTVVDKAVSSGIVVAAAAGNEGSSG
STSTVGYPAKYPSTIAVGAVNSSNQRASFSSVGSELDVMAPGVSIQSTLPGGTYGAYNGT(SEB)MATPHVAGAAALILS
KHPTWTNAQVRDRLESTATYLGNSFYYGKGLINVQAAAQ

AQSVPYGISQIKAPALHSQGYTGSNVKVAVIDSGIDSSHPDLNVRGGASFVPSETNPYQDGSSHGTHVAGTIAALNNSIG
VLGVAPSASLYAVKVLDSTGSGQYSWIINGIEWAISNNMDVINMSLGGPTGSTALKTVVDKAVSSGIVVAAAAGNEGSSG
STSTVGYPAKYPSTIAVGAVNSSNQRASFSSVGSELDVMAPGVSIQSTLPGGTYGAYNGTSMATPHVAGAAALILSKHPT
WTNAQVRDRLESTATYLGNSFYYGKGLINVQAAAQ

6.4mg/ml Nattokinase, 50mM Tris HCl, 50mM HEPES, 10% PEG 8000, 8% ethylene glycol, 5mM CaCl2, 100mM NaCl, 2mM PMSF, 4mM Pefabloc SC, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K