Crystal structure of human OTUB1/UbcH5b~Ub/Ub

OTUB1 Co-opts Lys48-Linked Ubiquitin Recognition to Suppress E2 Enzyme Function.

Ubiquitin-conjugating enzyme E2 D2, Ubiquitin thioesterase OTUB1

Ubiquitin carrier protein D2, Ubiquitin-conjugating enzyme E2(17)KB 2, Ubiquitin-conjugating enzyme E2-17 kDa 2, Ubiquitin-protein ligase D2, Deubiquitinating enzyme OTUB1, OTU domain-containing ubiquitin aldehyde-binding protein 1, Otubain-1, hOTU1, Ubiquitin-specific-processing protease OTUB1

GAMALKRIHKELNDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNI
NSNGSISLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDREKYNRIAREWTQKYAMGGSAYDEAIMA
QQDRIQQEIAVQNPLVSERLELSVLYKEYAEDDNIYQQKIKDLHKKYSYIRKTRPDGNSFYRAFGFSHLEALLDDSKELQ
RFKAVSAKSKEDLVSQGFTEFTIEDFHNTFMDLIEQVEKQTSVADLLASFNDQSTSDYLVVYLRLLTSGYLQRESKFFEH
FIEGGRTVKEFCQQEVEPMCKESDHIHIIALAQALSVSIQVEYMDRGEGGTTNPHIFPEGSEPKVYLLYRPGHYDILYK

UBE2D2, UBC4, UBC5B, UBCH4, UBCH5B, HSPC263, OTB1, OTU1, OTUB1

25% PEG1500, 0.1 M SPG, pH 8.0, VAPOR DIFFUSION, temperature 293K

Ubiquitin thioesterase OTUB1 (E.C.6.3.2.19, 3.4.19.12), Ubiquitin-conjugating enzyme E2 D2, Polyubiquitin-C

There are three biological complexes per
asymmetric unit. The biological complex defined by the
author consists of
Complex 1: chain H, chain E, residues 1 to 147 of chain C,
and residues 1025-1071 of chain B;
Complex 2: chain G, chain D, residues 1 to 147 of chain B,
and residues 1025-1271 of chain a;
Complex 3: chain I, chain F, residues 1 to 147 of chain A,
and residues 1025-1271 of chain C;

chain 'A' and (resseq 1:150 ) and (not element H) and (not element D)

chain 'B' and (resseq 1:150 ) and (not element H) and (not element D)

chain 'C' and (resseq 1:150 ) and (not element H) and (not element D)

chain 'A' and (resseq 1025:1271 ) and (not element H) and (not element D)

chain 'B' and (resseq 1025:1271 ) and (not element H) and (not element D)

chain 'C' and (resseq 1025:1271 ) and (not element H) and (not element D)

MALKRIHKELNDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINS
NGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDREKYNRIAREWTQKYAM

AYDEAIMAQQDRIQQEIAVQNPLVSERLELSVLYKEYAEDDNIYQQKIKDLHKKYSYIRKTRPDGNCFYRAFGFSHLEAL
LDDSKELQRFKAVSAKSKEDLVSQGFTEFTIEDFHNTFMDLIEQVEKQTSVADLLASFNDQSTSDYLVVYLRLLTSGYLQ
RESKFFEHFIEGGRTVKEFCQQEVEPMCKESDHIHIIALAQALSVSIQVEYMDRGEGGTTNPHIFPEGSEPKVYLLYRPG
HYDILYK