Structure of bovine endothelial nitric oxide synthase heme domain in complex with (N1-(2-(1H-imidazol-1-yl)pyrimidin-4-yl)-N2-(3- fluorophenethyl)ethane-1,2-diamine

Novel 2,4-Disubstituted Pyrimidines as Potent, Selective, and Cell-Permeable Inhibitors of Neuronal Nitric Oxide Synthase.

CONSTITUTIVE NOS, CNOS, EC-NOS, ENDOTHELIAL NOS, ENOS, NOS TYPE III, NOSIII, ENDOTHELIAL NITRIC OXIDE SYNTHASE

RAPAPATPHAPDHSPAPNSPTLTRPPEGPKFPRVKNWELGSITYDTLCAQSQQDGPCTPRRCLGSLVLPRKLQTRPSPGP
PPAEQLLSQARDFINQYYSSIKRSGSQAHEERLQEVEAEVASTGTYHLRESELVFGAKQAWRNAPRCVGRIQWGKLQVFD
ARDCSSAQEMFTYICNHIKYATNRGNLRSAITVFPQRAPGRGDFRIWNSQLVRYAGYRQQDGSVRGDPANVEITELCIQH
GWTPGNGRFDVLPLLLQAPDEAPELFVLPPELVLEVPLEHPTLEWFAALGLRWYALPAVSNMLLEIGGLEFSAAPFSGWY
MSTEIGTRNLCDPHRYNILEDVAVCMDLDTRTTSSLWKDKAAVEINLAVLHSFQLAKVTIVDHHAATVSFMKHLDNEQKA
RGGCPADWAWIVPPISGSLTPVFHQEMVNYILSPAFRYQPDPW

20-22% PEG3350, 0.1 M CACODYLATE, 150-200 MM MG ACETATE, 5 MM TCEP, pH 6.0

STRUCTURE OF RAT NEURONAL NITRIC OXIDE SYNTHASE HEME DOMAIN IN COMPLEX WITH N-2-(2-(1H-IMIDAZOL-1-YL) PYRIMIDIN-4-YL)ETHYL-(S,R)-2-(3-FLUOROBENZYL) CYCLOPROPAN-1-AMINE

STRUCTURE OF RAT NEURONAL NITRIC OXIDE SYNTHASE HEME DOMAIN IN COMPLEX WITH N-2-(2-(1H-IMIDAZOL-1-YL) PYRIMIDIN-4-YL)ETHYL-(R,S)-2-(3-FLUOROBENZYL) CYCLOPROPAN-1-AMINE

STRUCTURE OF RAT NEURONAL NITRIC OXIDE SYNTHASE HEME DOMAIN IN COMPLEX WITH N-2-(2-(1H-IMIDAZOL-1-YL) PYRIMIDIN-4-YL)ETHYL-3-(PYRIDIN-3-YL)PROPAN-1- AMINE

STRUCTURE OF RAT NEURONAL NITRIC OXIDE SYNTHASE HEME DOMAIN IN COMPLEX WITH 2-(2-(1H-IMIDAZOL-1-YL) PYRIMIDIN-4-YL)-N-(3-CYANOBENZYL)ETHAN-1-AMINE

STRUCTURE OF RAT NEURONAL NITRIC OXIDE SYNTHASE HEME DOMAIN IN COMPLEX WITH 3-(3-FLUOROPHENYL)-N-2-(2-( 5-METHYL-1H-IMIDAZOL-1-YL)PYRIMIDIN-4-YL)ETHYLPROPAN -1-AMINE

STRUCTURE OF BOVINE ENDOTHELIAL NITRIC OXIDE SYNTHASE HEME DOMAIN IN COMPLEX WITH 2-(2-(1H-IMIDAZOL-1-YL )PYRIMIDIN-4-YL)-N-(3-FLUOROPHENETHYL)ETHAN-1-AMINE

STRUCTURE OF BOVINE ENDOTHELIAL NITRIC OXIDE SYNTHASE HEME DOMAIN IN COMPLEX WITH N-2-(2-(1H-IMIDAZOL-1 -YL)PYRIMIDIN-4-YL)ETHYL-3-(3-FLUOROPHENYL)PROPAN-1 -AMINE

STRUCTURE OF BOVINE ENDOTHELIAL NITRIC OXIDE SYNTHASE HEME DOMAIN IN COMPLEX WITH N-2-(2-(1H-IMIDAZOL-1 -YL)PYRIMIDIN-4-YL)ETHYL-3-(3-CHLOROPHENYL)PROPAN-1 -AMINE

STRUCTURE OF BOVINE ENDOTHELIAL NITRIC OXIDE SYNTHASE HEME DOMAIN IN COMPLEX WITHN-{[(1R,2R)-2-(3- FLUOROPHENYL)CYCLOPROPYL]METHYL}-2-[2-(1H-IMIDAZOL-1- YL)PYRIMIDIN-4-YL]ETHANAMINE

STRUCTURE OF BOVINE ENDOTHELIAL NITRIC OXIDE SYNTHASE HEME DOMAIN IN COMPLEX WITH N-2-(2-(1H-IMIDAZOL-1 -YL)PYRIMIDIN-4-YL)ETHYL-(S,R)-2-(3-FLUOROBENZYL) CYCLOPROPAN-1-AMINE

STRUCTURE OF BOVINE ENDOTHELIAL NITRIC OXIDE SYNTHASE HEME DOMAIN IN COMPLEX WITH2-(2-(1H-IMIDAZOL-1-YL) PYRIMIDIN-4-YL)-N-(3-CYANOBENZYL)ETHAN-1-AMINE

STRUCTURE OF BOVINE ENDOTHELIAL NITRIC OXIDE SYNTHASE HEME DOMAIN IN COMPLEX WITH 3-(3-FLUOROPHENYL)-N-2 -(2-(5-METHYL-1H-IMIDAZOL-1-YL)PYRIMIDIN-4-YL) ETHYLPROPAN-1-AMINE

STRUCTURE OF RAT NEURONAL NITRIC OXIDE SYNTHASE HEME DOMAIN IN COMPLEX WITH N1-(2-(1H-IMIDAZOL-1-YL) PYRIMIDIN-4-YL)-N2-(3-FLUOROPHENETHYL)ETHANE-1,2- DIAMINE

STRUCTURE OF HUMAN NNOS R354A G357D MUTANT HEME DOMAIN IN COMPLEX WITH N-2-(2-(1H-IMIDAZOL-1-YL) PYRIMIDIN-4-YL)ETHYL-3-(PYRIDIN-3-YL)PROPAN-1- AMINE

STRUCTURE OF RAT NEURONAL NITRIC OXIDE SYNTHASE HEME DOMAIN IN COMPLEX WITH N-(2-(1H-IMIDAZOL-1-YL)-4- PYRIMIDYLMETHYL)-3-(3-FLUOROPHENYL)PROPAN-1-AMINE

STRUCTURE OF RAT NEURONAL NITRIC OXIDE SYNTHASE HEME DOMAIN IN COMPLEX WITH 2-(2-(1H-IMIDAZOL-1-YL) PYRIMIDIN-4-YL)-N-(3-FLUOROPHENETHYL)ETHAN-1-AMINE

STRUCTURE OF RAT NEURONAL NITRIC OXIDE SYNTHASE HEME DOMAIN IN COMPLEX WITH N-2-(2-(1H-IMIDAZOL-1-YL) PYRIMIDIN-4-YL)ETHYL-3-(3-FLUOROPHENYL)PROPAN-1- AMINE

STRUCTURE OF RAT NEURONAL NITRIC OXIDE SYNTHASE HEME DOMAIN IN COMPLEX WITH N-2-(2-(1H-IMIDAZOL-1-YL) PYRIMIDIN-4-YL)ETHYL-3-(3-CHLOROPHENYL)PROPAN-1- AMINE

STRUCTURE OF RAT NEURONAL NITRIC OXIDE SYNTHASE HEME DOMAIN IN COMPLEX WITH 2-(2-(1H-IMIDAZOL-1-YL) PYRIMIDIN-4-YL)-N-(2-(3-FLUOROPHENYL)CYCLOPROPYLMETHYL) ETHAN-1-AMINE

RESIDUE 100 IS FOUND AS AN ARG IN STRUCTURE BUT IS A CYS IN DATABASE

RESIDUES 110 TO 120 IN BOTH CHAIN A AND CHAIN B ARE DISORDERED.

NITRIC OXIDE SYNTHASE, ENDOTHELIAL (E.C.1.14.13.39)

OXIDOREDUCTASE, NITRIC OXIDE SYNTHASE, INHIBITOR COMPLEX