Regulation of the mammalian elongation cycle by 40S subunit rolling: a eukaryotic-specific ribosome rearrangement

Regulation of the Mammalian Elongation Cycle by Subunit Rolling: A Eukaryotic-Specific Ribosome Rearrangement.

SUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD -2624. (DEPOSITION ID: 12412).
CROSS- -CORRELATION WITH KNOWN STRUCTURE

20 MM HEPES, 5 MM MGCL2, 100 MM NH4CL, 6 MM BETA- MERCAPTOETHANOL, 0.8 MM SPERMIDINE, 0.6 MM SPERMINE

CODON RECOGNITION - GTPASE ACTIVATION STATE OF MAMMALIAN 80S RIBOSOME

VITRIFICATION 1 -- CRYOGEN- ETHANE, HUMIDITY- 80, INSTRUMENT - FEI VITROBOT MARK II, METHOD- BLOT FOR 2 SEC BEFORE PLUNGING,

EF-1-ALPHA, ELONGATION FACTOR TU, EF-TU, ELONGATION FACTOR 1A

CGCCCGUCGCUACUACCGAUUGGAUGGUUUAGUGAGGCCCUCGGAUCGGCCCCGCCGGGGUCGGCCCACGGCCCUGGCGG
AGCGCUGAGAAGACGGUCGAACUUGACUAUCUAGAGGAAGUAAAAGUCGUAACAA

MAEKPHMNLVVIGHVDHGKSTLVGHLLYRLGYIEEKKLKELEEQAKSRGKESFKFAWILDKMKEERERGITIDLTFMKFE
TKKYVFTIIDAPGHRDFVKNMITGASQADAAILVVSARKGEFEAGMSTEGQTREHLLLARTMGIEQIIVAVNKMDAPDVN
YDQKRYEFVVSVLKKFMKGLGYQVDKIPFIPVSAWKGDNLIERSPNMPWYNGPTLVEALDQLQPPAKPVDKPLRIPVQNV
YSIPGAGTVPVGRVETGVLRVGDKVVFMPPGVVGEVRSIEMHYQQLQQAEPGDNIGFAVRGVSKSDIKRGDVAGHLDKPP
TVAEEFEARIFVIWHPSAITVGYTPVIHVHTASVSSRIIEIKAKLDPKTGQVVEQNPQFLKAGDAAIVRFKPVKPLVVEK
FSEIPQLGRFAMRDMNRTVGIGIVTDVKPAKVDIKAK

MGKCRGLRTARKLRSHRRDQKWHDKQYKKAHLGTALKANPFGGASHAKGIVLEKVGVEAKQPNSAIRKCVRVQLIKNGKK
ITAFVPNDGCLNFIEENDEVLVAGFGRKGHAVGDIPGVRFKVVKVANVSLLALYKGKKERPRS

GCGGAUUUA(2MG)CUCAG(H2U)(H2U)GGGAGAGC(M2G)CCGGU(OMC)UCCA(MIA)AACCGGAG(7MG)UC
(5MC)UGUG(5MU)(PSU)CG(1MA)UCCACAGAAUUCGCACCA

FOR MODELING MRNA (CHAIN 1, PDB ID CODE 3I8G) WAS USED

SHEET
DETERMINATION METHOD: DSSP
THE SHEETS PRESENTED AS "XA" IN EACH CHAIN ON SHEET RECORDS
BELOW IS ACTUALLY AN 5-STRANDED BARREL THIS IS REPRESENTED BY
A 6-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS
ARE IDENTICAL.

ELONGATION FACTOR 1A, 40S RIBOSOMAL PROTEIN US12/RNA Complex, PHENYLALANINE

TRANSLATION, MAMMALIAN 80S RIBOSOME, ELONGATION CYCLE, TRNA SELECTION, EUKARYOTIC TERNARY COMPLEX, ELONGATION FACTOR EEF1A