Structure of bovine endothelial nitric oxide synthase heme domain in complex with (R)-6-(3-amino-2-(5-(2-(6-amino-4-methylpyridin-2-yl) ethyl)pyridin-3-yl)propyl)-4-methylpyridin-2-amine

Nitric Oxide Synthase Inhibitors that Interact with Both a Heme Propionate and Tetrahydrobiopterin Show High Isoform Selectivity.

CONSTITUTIVE NOS, CNOS, EC-NOS, ENDOTHELIAL NOS, ENOS, NOS TYPE III, NOSIII, ENDOTHELIAL NITRIC OXIDE SYNTHASE

RAPAPATPHAPDHSPAPNSPTLTRPPEGPKFPRVKNWELGSITYDTLCAQSQQDGPCTPRRCLGSLVLPRKLQTRPSPGP
PPAEQLLSQARDFINQYYSSIKRSGSQAHEERLQEVEAEVASTGTYHLRESELVFGAKQAWRNAPRCVGRIQWGKLQVFD
ARDCSSAQEMFTYICNHIKYATNRGNLRSAITVFPQRAPGRGDFRIWNSQLVRYAGYRQQDGSVRGDPANVEITELCIQH
GWTPGNGRFDVLPLLLQAPDEAPELFVLPPELVLEVPLEHPTLEWFAALGLRWYALPAVSNMLLEIGGLEFSAAPFSGWY
MSTEIGTRNLCDPHRYNILEDVAV(CAS)MDLDTRTTSSLWKDKAAVEINLAVLHSFQLAKVTIVDHHAATVSFMKHLDN
EQKARGGCPADWAWIVPPISGSLTPVFHQEMVNYILSPAFRYQPDPW

RAPAPATPHAPDHSPAPNSPTLTRPPEGPKFPRVKNWELGSITYDTLCAQSQQDGPCTPRRCLGSLVLPRKLQTRPSPGP
PPAEQLLSQARDFINQYYSSIKRSGSQAHEERLQEVEAEVASTGTYHLRESELVFGAKQAWRNAPRCVGRIQWGKLQVFD
ARDCSSAQEMFTYICNHIKYATNRGNLRSAITVFPQRAPGRGDFRIWNSQLVRYAGYRQQDGSVRGDPANVEITELCIQH
GWTPGNGRFDVLPLLLQAPDEAPELFVLPPELVLEVPLEHPTLEWFAALGLRWYALPAVSNMLLEIGGLEFSAAPFSGWY
MSTEIGTRNLCDPHRYNILEDVAVCMDLDTRTTSSLWKDKAAVEINLAVLHSFQLAKVTIVDHHAATVSFMKHLDNEQKA
RGGCPADWAWIVPPISGSLTPVFHQEMVNYILSPAFRYQPDPW

20-22% PEG3350, 0.1 M CACODYLATE, 150-200 MM MG ACETATE, 5 MM TCEP, pH 6.0

STRUCTURE OF RAT NEURONAL NITRIC OXIDE SYNTHASE HEME DOMAIN IN COMPLEX WITH (S)-6-(2-AMINO-2-(3-(2-(6 -AMINO-4-METHYLPYRIDIN-2-YL)ETHYL)PHENYL)ETHYL)-4- METHYLPYRIDIN-2-AMINE

STRUCTURE OF RAT NEURONAL NITRIC OXIDE SYNTHASE HEME DOMAIN IN COMPLEX WITH (R)-6-(2-AMINO-2-(3-(2-(6 -AMINO-4-METHYLPYRIDIN-2-YL)ETHYL)PHENYL)ETHYL)-4- METHYLPYRIDIN-2-AMINE

STRUCTURE OF RAT NEURONAL NITRIC OXIDE SYNTHASE HEME DOMAIN IN COMPLEX WITH 2-(6-AMINO-4-METHYLPYRIDIN-2 -YL)-1-(3-(2-(6-AMINO-4-METHYLPYRIDIN-2-YL)ETHYL )PHENYL)ETHAN-1-OL

STRUCTURE OF RAT NEURONAL NITRIC OXIDE SYNTHASE HEME DOMAIN IN COMPLEX WITH (S)-2-(6-(2,5-DIMETHYL-1H- PYRROL-1-YL)-4-METHYLPYRIDIN-2-YL)-1-(3-(2-(4- METHYLPYRIDIN-2-YL)ETHYL)PHENYL)ETHAN-1-AMINE

STRUCTURE OF RAT NEURONAL NITRIC OXIDE SYNTHASE HEME DOMAIN IN COMPLEX WITH 6-(3-AMINO-2-(3-(2-(6- AMINO-4-METHYLPYRIDIN-2-YL)ETHYL)PHENYL)PROPYL)-4- METHYLPYRIDIN-2-AMINE

STRUCTURE OF RAT NEURONAL NITRIC OXIDE SYNTHASE HEME DOMAIN IN COMPLEX WITH 6-(3-AMINO-2-(6-(2-(6- AMINO-4-METHYLPYRIDIN-2-YL)ETHYL)PYRIDIN-2-YL)PROPYL )-4-METHYLPYRIDIN-2-AMINE

STRUCTURE OF RAT NEURONAL NITRIC OXIDE SYNTHASE HEME DOMAIN IN COMPLEX WITH (R)-6-(3-AMINO-2-(5-(2-(6 -AMINO-4-METHYLPYRIDIN-2-YL)ETHYL)PYRIDIN-3-YL) PROPYL)-4-METHYLPYRIDIN-2-AMINE

STRUCTURE OF RAT NEURONAL NITRIC OXIDE SYNTHASE HEME DOMAIN IN COMPLEX WITH (S)-6-(3-AMINO-2-(5-(2-(6 -AMINO-4-METHYLPYRIDIN-2-YL)ETHYL)PYRIDIN-3-YL) PROPYL)-4-METHYLPYRIDIN-2-AMINE

STRUCTURE OF BOVINE ENDOTHELIAL NITRIC OXIDE SYNTHASE HEME DOMAIN IN COMPLEX WITH (R)-6-(2-AMINO-2-(3-( 2-(6-AMINO-4-METHYLPYRIDIN-2-YL)ETHYL)PHENYL)ETHYL )-4-METHYLPYRIDIN-2-AMINE

STRUCTURE OF BOVINE ENDOTHELIAL NITRIC OXIDE SYNTHASE HEME DOMAIN IN COMPLEX WITH (S)-2-(6-(2,5-DIMETHYL -1H-PYRROL-1-YL)-4-METHYLPYRIDIN-2-YL)-1-(3-(2-( 4-METHYLPYRIDIN-2-YL)ETHYL)PHENYL)ETHAN-1-AMINE

STRUCTURE OF BOVINE ENDOTHELIAL NITRIC OXIDE SYNTHASE HEME DOMAIN IN COMPLEX WITH (S)-6-(3-AMINO-2-(5-( 2-(6-AMINO-4-METHYLPYRIDIN-2-YL)ETHYL)PYRIDIN-3-YL )PROPYL)-4-METHYLPYRIDIN-2-AMINE

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. U VALUES WITH TLS ADDED RESIDUES 110 TO 120 IN BOTH CHAIN A AND CHAIN B ARE DISORDERED.

NITRIC OXIDE SYNTHASE, ENDOTHELIAL (E.C.1.14.13.39)