Structure of the amidase domain of allophanate hydrolase from Pseudomonas sp strain ADP

X-Ray Structure of the Amidase Domain of Atzf, the Allophanate Hydrolase from the Cyanuric Acid-Mineralizing Multienzyme Complex.

THE C-TERMINAL DOMAIN WAS REMOVED FOR CRYSTALLIZATION OF THE PROTEIN

MGSSHHHHHHSSGLVPRGSHMNDRAPHPERSGRVTPDHLTDLASYQAAYAAGTDAADVISDLYARIKEDGENPIWISLLP
LESALAMLADAQQRKDKGEALPLFGIPFGVKDNIDVAGLPTTAGCTGFARTPRQHAFVVQRLVDAGAIPIGKTNLDQFAT
GLNGTRTPFGIPRCVFNENYVSGGSSSGSAVAVANGTVPFSLGTDTAGSGRIPAAFNNLVGLKPTKGLFSGSGLVPAARS
LDCISVLAHTVDDALAVARVAAGYDADDAFSRKAGAAALTEKSWPRRFNFGVPAAEHRQFFGDAEAEALFNKAVRKLEEM
GGTCISFDYTPFRQAAELLYAGPWVAERLAAIESLADEHPEVLHPVVRDIILSAKRMSAVDTFNGIYRLADLVRAAESTW
EKIDVMLLPTAPTIYTVEDMLADPVRLNSNLGFYTNFVNLMDLSAIAVPAGFRTNGLPFGVTFIGRAFEDGAIASLGKAF
VEHDLAK

PROTEIN AT 5.5 MG/ML WAS ADDED TO THE RESERVOIR CONDITION OF 11-14% (W/V) PEG 3350, 2% TACSIMATE PH 5. MICROSEEDS WERE ALSO ADDED TO THESE DROPS FOR REPRODUCIBLE CRYSTALS.

A HIS-TAG WAS ADDED TO THE N-TERMINUS AND THE C-TERMINAL DOMAIN WAS REMOVED FOR CRYSTALLIZATION OF THE PROTEIN.

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. U VALUES REFINED INDIVIDUALLY