RRM domain from C. elegans SUP-12 bound to GGTGTGC DNA
Backbone-Independent Nucleic Acid Binding by Splicing Factor Sup-12 Reveals Key Aspects of Molecular Recognition
THE HEPTAMER SEQUENCE IS DERIVED FROM THE INTRON BETWEEN EXONS 4 AND 5B FROM THE C. ELEGANS EGL-15 GENE.
GAMGSRDTMFTKIFVGGLPYHTSDKTLHEYFEQFGDIEEAVVITDRNTQKSRGYGFVTMKDRASAERACKDPNPIIDGRK ANVNLAYLGAKPRTNVQ
THE SAMPLE CONTAINS AN ADDITIONAL GLY-ALA-MET- AT THE N- TERMINUS FOLLOWING CLEAVAGE BY TEV PROTEASE
THE STRUCTURE WAS DETERMINED BY USING MULTI- DIMENSIONAL NMR SPECTRSOCOPY WITH 15N-, 13C,15N-, OR 2H- LABELED SUP-12 IN COMPLEX WITH A NATURAL ABUNDANCE GGTGTGC DNA LIGAND, OR A LIGAND WITH SITE-SPECIFIC 13C,15N- LABELING.
BRUNGER,ADAMS,CLORE,DELANO,GROS,GROSSE- KUNSTLEVE,JIANG,KUSZEWSKI,NILGES,PANNU,READ, RICE,SIMONSON,WARREN
TRANSCRIPTION-DNA COMPLEX, MUSCLE, DEVELOPMENT