Crystal structure of eIF4AIII-CWC22 complex

Crystal Structure of the Human Eif4Aiii-Cwc22 Complex Shows How a Dead-Box Protein is Inhibited by a Mif4G Domain

EIF-4A-III, EIF4A-III, ATP-DEPENDENT RNA HELICASE DDX48, ATP-DEPENDENT RNA HELICASE EIF4A-3, DEAD BOX PROTEIN 48, EUKARYOTIC INITIATION FACTOR 4A-LIKE NUK-34, EUKARYOTIC TRANSLATION INITIATION FACTOR 4A ISOFORM 3, NUCLEAR MATRIX PROTEIN 265, NMP 265, HNMP 265, EUKARYOTIC INITIATION FACTOR 4A-III, N-TERMINALLY PROCESSED

PRE-MRNA-SPLICING FACTOR CWC22, NUCAMPHOLIN HOMOLOG, FSAPB

MATTATMATSGSARKRLLKEEDMTKVEFETSEEVDVTPTFDTMGLREDLLRGIYAYGFEKPSAIQQRAIKQIIKGRDVIA
QSQSGTGKTATFSISVLQCLDIQVRETQALILAPTRELAVQIQKGLLALGDYMNVQCHACIGGTNVGEDIRKLDYGQHVV
AGTPGRVFDMIRRRSLRTRAIKMLVLDEADEMLNKGFKEQIYDVYRYLPPATQVVLISATLPHEILEMTNKFMTDPIRIL
VKRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVDWLTEKMREANFTVSSMHGDMPQKERESIM
KEFRSGASRVLISTDVWARGLDVPQVSLIINYDLPNNRELYIHRIGRSGRYGRKGVAINFVKNDDIRILRDIEQYYSTQI
DEMPMNVADLI

KKKKDELDPLLTRTGGAYIPPAKLRMMQEQITDKNSLAYQRMSWEALKKSINGLINKVNISNISIIIQELLQENIVRGRG
LLSRSVLQAQSASPIFTHVYAALVAIINSKFPQIGELILKRLILNFRKGYRRNDKQLCLTASKFVAHLINQNVAHEVLCL
EMLTLLLERPTDDSVEVAIGFLKECGLKLTQVSPRGINAIFERLRNILHESEIDKRVQYMIEVMFAVRKDGFKDHPIILE
GLDLVEEDDQFTHMLPLEDDYNPEDVLNVFKMDPNFMENEEKYKAIKKEIL

12% PEG 20000, 0.1 M NA-K-PHOSPHATE PH 6.5