Crystal structure of the Sonic Hedgehog-heparin complex

O2-SULFO-GLUCURONIC ACID; 2-O-sulfo-alpha-L-iduronic acid; 2-O-sulfo-L-iduronic acid; 2-O-sulfo-iduronic acid

N,O6-DISULFO-GLUCOSAMINE; 6-O-sulfo-N-sulfo-alpha-D-glucosamine; 2-deoxy-6-O-sulfo-2-(sulfoamino)-alpha-D-glucose; 2-deoxy-6-O-sulfo-2-(sulfoamino)-D-glucose; 2-deoxy-6-O-sulfo-2-(sulfoamino)-glucose

Structural Insights Into Proteoglycan-Shaped Hedgehog Signaling.

N-TEMINAL SIGNALLING DOMAIN, RESIDUES 40-195

2-deoxy-6-O-sulfo-2-(sulfoamino)-alpha-D-glucopyranose-(1-4)-2-O-sulfo-alpha-L-idopyranuronic acid-(1-4)-2-deoxy-6-O-sulfo-2-(sulfoamino)-alpha-D-glucopyranose-(1-4)-2-O-sulfo-alpha-L-idopyranuronic acid-(1-4)-2-deoxy-6-O-sulfo-2-(sulfoamino)-alpha-D-glucopyranose-(1-4)-2-O-sulfo-alpha-L-idopyranuronic acid

2-O-sulfo-alpha-L-idopyranuronic acid-(1-4)-2-deoxy-6-O-sulfo-2-(sulfoamino)-alpha-D-glucopyranose-(1-4)-2-O-sulfo-alpha-L-idopyranuronic acid-(1-4)-2-deoxy-6-O-sulfo-2-(sulfoamino)-alpha-D-glucopyranose-(1-4)-2-O-sulfo-alpha-L-idopyranuronic acid-(1-4)-2-deoxy-6-O-sulfo-2-(sulfoamino)-alpha-D-glucopyranose

MALTPLAYKQFIPNVAEKTLGASGRYEGKITRNSERFKELTPNYNPDIIFKDEENTGADRLMTQRCKDKLNALAISVMNQ
WPGVKLRVTEGWDEDGHHSEESLHYEGRAVDITTSDRDRSKYGMLARLAVEAGFDWVYYESKAHIHCSVKAENSVAAKHH
HHHH

100 MM TRIS-HCL PH 8.5; 200 MM SODIUM ACETATE; 30% (W/V) PEG 4000; 8% (V/V) 1,1,1,3,3, 3-HEXAFLUORO-2-PROPANOL

CRYSTAL STRUCTURE OF THE SONIC HEDGEHOG-CHONDROITIN-4- SULPHATE COMPLEX

2-O-SULFO-ALPHA-L-IDOPYRANURONIC ACID (IDS): THE
OLIGOSACCHARIDE CHAIN IDS-SGN IS CONTINUOUS RUNNING
THROUGH SEVERAL UNIT CELLS.
N,O6-DISULFO-GLUCOSAMINE (SGN): THE OLIGOSACCHARIDE CHAIN
IDS-SGN IS CONTINUOUS RUNNING THROUGH SEVERAL UNIT CELLS.

CONSTRUCT CONTAINS TWO ADDITIONAL N-TERMINAL RESIDUES (MA) AND 6 ADDITIONAL C-TERMINAL RESIDUES (HHHHHH)

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. THE OLIGOSACCHARIDE CHAIN IDS-SGN IS PRESENT IN TWO DISTINCT CONFORMATIONS WHICH ARE SYMMETRY MATES. ONLY ONE CONFORMATION IS PRESENT IN THE PDB FILE WITH 0.50 OCCCUPANCY, SINCE THE OTHER CAN BE GENERATED BY APPLYING THE SYMMETRY OPERATIONS OF THE SPACE GROUP.

SIGNALING PROTEIN, HEDGEHOG SIGNALLING, MORPHOGENS, HEPARAN SULPHATE PROTEOGLYCANS, GLYCOSAMINOGLYCANS