Cryo-EM structure of Dengue virus serotype 1 complexed with Fab fragments of human antibody 1F4

N-acetyl-beta-D-glucosamine; 2-acetamido-2-deoxy-beta-D-glucose; 2-acetamido-2-deoxy-D-glucose; 2-acetamido-2-deoxy-glucose; N-ACETYL-D-GLUCOSAMINE

A Potent Anti-Dengue Human Antibody Preferentially Recognizes the Conformation of E Protein Monomers Assembled on the Virus Surface.

METHOD--RIGID BODY REFINEMENT PROTOCOL--CRYO-EM

SUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD-2442. (DEPOSITION ID: 11902).

12 MM TRIS-HCL PH 8.0, 120 MM NACL AND 1 MM EDTA

DENGUE VIRUS SEROTYPE 1 WITH FAB FRAGMENTS OF HUMAN MONOCLONAL ANTIBODY 1F4

VITRIFICATION 1 -- CRYOGEN- ETHANE, HUMIDITY- 100, TEMPERATURE- 100, INSTRUMENT- FEI VITROBOT MARK IV, METHOD- BLOT WITH FILTER PAPER FOR 2 S BEFORE PLUNGING,

HEAVY CHAIN FAB FRAGMENT OF ANTIBODY 1F4

LIGHT CHAIN FAB FRAGMENT OF ANTIBODY 1F4

MRCVGISNRDFVEGLSATGWVDVVLEHGSCVTTMAKDKPTLDIELLKTEVTNPAVLRKLCIEAKISNTTTDSRCPTQGEA
TLVEEQDANFVCRRTFVDRGWGNGCGLFGKGSLITCAKFKCVTKLEGKIVQYENLKYSVIVTVHTGDQHQVGNESTEHGT
TATITPQAPTTEIQLTDYGALTLDCSPRTGLDFNEMVLLTMKEKSWLVHKQWFLDLPLPWTSGASTSQETWNRQDLLVTF
KTAHAKKQEVVVLGSQEGAMHTALTGATEIQTSGTTTIFAGHLKCRLKMDKLTLKGMSYVMCTGSFKLEKEVAETQHGTV
LVQIKYEGTDAPCKIPFSTQDEKGVTQNGRLITANPIVTDKEKPVNIEAEPPFGESYIVIGAGEKALKLSWFKKGSSIGK
MFEATARGARRMAILGDTAWDFGSIGGVFTSVGKLVHQIFGTAYGVLFSGVSWTMKIGIGVLLTWLGLNSRSTSLSMTCI
AVGLVTLYLGVMVQA

GSWAQVQLVESGGGVVQPGRSLRLSCAASGFTFSAYGMHWVRQAPGKGLEWVAIIWYDGSNKYYADSVKGRFTISRDNSK
NTLHLQMNSLRAEDTAIYYCARDKNPGTKPYYHYGMDVWGQGTTVTVSSAGTKGPSVFPLAPSSKSTSGGTAALGCLVKD
YFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSC

LATMAWMMLLLLTLITHCAGSWAQSVLTQPPSVSEAPRQRVTISCSGSSSNIGNNAVNWYQQFPGKAPKLLIYYDDLLPS
GVSDRFSGSKSGTSASLAISGLQSEDEAYYYCAAWDDSLIGVVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLV
CLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS

DENGUE VIRUS SEROTYPE 1 STRAIN PVP159 USED IN THIS STRUCTURAL STUDY IS A LABORATORY STRAIN. IT HAS SEVERAL MUTATIONS COMPARED TO THAT DESCRIBED IN Q7TGE4. IT HAS BEEN SEQUENCED AS A PART OF POLYPROTEIN ENCODED BY THE VIRAL GENOME.

icosahedral asymmetric unit, std point frame

CA ATOMS ONLY, CHAIN A, C, E, B, D, F, H, M, L, N