Arf1(Delta1-17)in complex with BRAG2 Sec7-PH domain

Integrated conformational and lipid-sensing regulation of endosomal ArfGEF BRAG2.

KIRKPATRICK-BAEZ PAIR OF BI-MORPH MIRRORS

CRYOGENICALLY COOLED MONOCHROMATOR CRYSTAL

IQ MOTIF AND SEC7 DOMAIN-CONTAINING PROTEIN 1

ADP-RIBOSYLATION FACTORS GUANINE NUCLEOTIDE-EXCHANGE PROTEIN 100, ADP-RIBOSYLATION FACTORS GUANINE NUCLEOTIDE-EXCHANGE PROTEIN 2, BREFELDIN-RESISTANT ARF-GEF 2 PROTEIN, BRAG2

GAMGSGIPMSWDSPAFSNDVIRKRHYRIGLNLFNKKPEKGVQYLIERGFVPDTPVGVAHFLLQRKGLSRQMIGEFLGNRQ
KQFNRDVLDCVVDEMDFSTMELDEALRKFQAHIRVQGKAQKVERLIEAFSQRYCICNPGVVRQFRNPDTIFILAFAIILL
NTDMYSPNVKPERKMKLEDFIKNLRGVDDGEDIPREMLMGIYERIRKRELKTNEDHVSQVQKVEKLIVGKKPIGSLHPGL
GCVLSLPHRRLVCYCRLFEVPDPNKPQKLGLHQREIFLFNDLLVVTKIFQKKKNSVTYSFRQSFSLYGMQVLLFENQYYP
NGIRLTSSVPGADIKVLINFNAPNPQDRKKFTDDLRESIAEVQEMEKHRIESELEKQKGVVRP

MRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDR
ERVNEAREELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRHRNWYIQATCATSGDGLYEGLDWLSNQL
RNQK

0.15 M AMMONIUM SULFATE, 0.1 M MES PH 6 AND 16% PEG 4000

ARF1 WAS DELETED FROM ITS 17 FIRST AMINO ACIDS IN THE
STRUCTURE
ISOFORM 2 AND IN THE STRUCTURE SEQUENCE REMAINS A PART OF
THE CLEAVED 6-HISTAG GAMGSGIP

IDEAL-DIST CONTACT TERM CONTACT SETUP. ALL ATOMS HAVE CCP4 ATOM TYPE FROM LIBRARY