Cyanuric acid hydrolase: evolutionary innovation by structural concatenation.

MGSSHHHHHHSSGLVPRGSHMYHIDVFRIPCHSPGDTSGLEDLIETGRVAPADIVAVMGKTEGNGCVNDYTREYATAMLA
ACLGRHLQLPPHEVEKRVAFVMSGGTEGVLSPHHTVFARRPAIDAHRPAGKRLTLGIAFTRDFLPEEIGRHAQITETAGA
VKRAMRDAGIASIDDLHFVQVKCPLLTPAKIASARSRGCAPVTTDTYESMGYSRGASALGIALATEEVPSSMLVDESVLN
DWSLSSSLASASAGIELEHNVVIAIGMSEQATSELVIAHGVMSDAIDAASVRRTIESLGIRSDDEMDRIVNVFAKAEASP
DGVVRGMRHTMLSDSDINSTRHARAVTGAAIASVVGHGMVYVSGGAEHQGPAGGGPFAVIARA

GENE SYNTHESIZED BASED ON SEQUENCE FROM ACCESSION NO U66917

THE PROTEIN WAS AT 1 MG/ML AFTER DIALYSIS WITH HEPES BUFFER AND CYANURIC ACID, THIS WAS ADDED IN A 3:1 RATIO TO THE RESERVIOR SOLUTION OF 38% V/V PEG 400 AND 100 MM HEPES PH 6.6

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. U VALUES REFINED INDIVIDUALLY