Structure and Activity of the Peptidyl-Prolyl Isomerase Domain from the Histone Chaperone Fpr4 Towards Histone H3 Proline Isomerization
C-TERMINAL PEPTIDYL PROLYL CIS-TRANS ISOMERASE DOMAIN FROM YEAST FPR4P
PEPTIDYL PROLINE ISOMERASE (PPI) DOMAIN, RESIDUES 280-392
GAMAKPKTKLLEGGIIIEDRVTGKGPHAKKGTRVGMRYVGKLKNGKVFDKNTKGKPFVFKLGQGEVIKGWDIGVAGMAVG GERRIVIPAPYAYGKQALPGIPANSELTFDVKLVSMK
CONTAINS N-TERMINAL GLY-ALA-MET-ALA- RESIDUES FOLLOWING CLEAVAGE OF THE HIS6-TAG BY TEV PROTEAS
STRUCTURE CALCULATION USED A COMBINATION OF DISTANCE RESTRAINTS (UNAMBIGUOUS AND AMBIGUOUS), DIHEDRAL ANGLE RESTRAINTS, HYDROGEN-BOND RESTRAINTS AND RESIDUAL DIPOLAR COUPLING RESTRAINTS
BRUNGER,ADAMS,CLORE,DELANO,GROS,GROSSE- KUNSTLEVE,JIANG,KUSZEWSKI,NILGES,PANNU,READ, RICE,SIMONSON,WARREN
ISOMERASE, PROLINE ISOMERIZATION, FKBP, HISTONE CHAPERONE