Structure of wild type Pseudomonas aeruginosa FabF (KASII)

Structures of Pseudomonas Aeruginosa Beta-Keto-Acyl-(Acyl-Carrier-Protein) Synthase II (Fabf) and a C164Q Mutant Provide Templates for Antibacterial Drug Discovery and Identify a Buried Potassium Ion and a Ligand-Binding Site that is an Artefact of the Crystal Form

3-OXOACYL-[ACYL-CARRIER-PROTEIN] SYNTHASE 2

BETA-KETOACYL-ACYL-CARRIER-PROTEIN SYNTHASE II

MHHHHHHSSGVDLGTENLYFQSMSRRRVVITGMGMLSPLGLDVPSSWEGILAGRSGIAPIEHMDLSAYSTRFGGSVKGFN
VEEYLSAKEARKLDLFIQYGLAASFQAVRDSGLEVTDANRERIGVSMGSGIGGLTNIENNCRSLFEQGPRRISPFFVPGS
IINMVSGFLSIHLGLQGPNYALTTACTTGTHSIGMAARNIAYGEADVMVAGGSEMAACGLGLGGFGAARALSTRNDEPTR
ASRPWDRDRDGFVLSDGSGALVLEELEHARARGARIYAELVGFGMSGDAFHMTAPPEDGAGAARCMKNALRDAGLDPRQV
DYINAHGTSTPAGDIAEIAAVKSVFGEHAHALSMSSTKSMTGHLLGAAGAVEAIFSVLALRDQVAPPTINLDNPDEGCDL
DLVAHEAKPRKIDVALSNSFGFGGTNGTLVFRRFAD

0.2 M MAGNESIUM CHLORIDE, 0.1 M TRIS PH 7.0, 10% (W/V) PEG8000

HELIX
DETERMINATION METHOD: AUTHOR PROVIDED.

SHEET
DETERMINATION METHOD: AUTHOR PROVIDED.

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.