Molecular structure of a metal-independent bacterial glycosyltransferase that catalyzes the synthesis of histo-blood group A antigen

alpha-L-fucose; 6-deoxy-alpha-L-galactopyranose; L-fucose; fucose

beta-D-galactose; D-galactose; galactose

Structure of a Metal-Independent Bacterial Glycosyltransferase that Catalyzes the Synthesis of Histo-Blood Group a Antigen

BOGT - METAL-INDEPENDENT GLYCOSYLTRANSFERASE

MRIGILYICTGKYDIFWKDFYLSAERYFMQDQSFIIEYYVFTDSPKLYDEENNKHIHRIKQKNLGWPDNTLKRFHIFLRI
KEQLERETDYLFFFNANLLFTSPIGKEILPPSDSNGLLGTMHPGFYNKPNSEFTYERRDASTAYIPEGEGRYYYAGGLSG
GCTKAYLKLCTTICSWVDRDATNHIIPIWHDESLINKYFLDNPPAITLSPAYLYPEGWLLPFEPIILIRDKNKPQYGGHE
LLRRKN

BOGT6A (4 MG/ML) AND SACCHARIDE (10 MM) WERE INCUBATED OVERNIGHT AT 16 DEG C AND CRYSTALS WERE GROWN IN A WELL SOLUTION CONTAINING 20 % PEG 3350, 0.1 M BIS-TRIS PROPANE, PH 8.5 AND 0.2 M NA/K TARTRATE

MOLECULAR STRUCTURE OF A METAL-INDEPENDENT BACTERIAL GLYCOSYLTRANSFERASE THAT CATALYZES THE SYNTHESIS OF HISTO -BLOOD GROUP A ANTIGEN