Negative stain EM Map of the AAA protein CbbX, a red-type Rubisco activase from R. sphaeroides

Structure and Function of the Aaa+ Protein Cbbx, a Red-Type Rubisco Activase.

METHOD--RIGID BODY REFINEMENT PROTOCOL--MANUAL

MODULE CONSISTING OF CHAIN A (204-296) AND CHAIN B (8-203) AS FOUND IN PDB 3SYL WAS MODELLED ONTO D2 P97 OF PDB 3CF3. SUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD-1932.

20 MM TRIS PH 8.0, 50 MM NACL, 5MM MGCL2, 1MM RIBULOSE-1,5- BISPHOSPHATE, 1MM ATP/ATPGAMMAS

THE PROTEIN IS BOUND TO RIBULOSE-1,5-BISPHOSPHATE

PTSIDLRAEYEGSGAKEVLEELDRELIGLKPVKDRIRETAALLLVERARQKLGLAHETPTLHMSFTGNPGTGKTTVALKM
AGLLHRLGYVRKGHLVSVTRDDLVGQYIGHTAPKTKEVLKRAMGGVLFIDEAYYLYRPDNERDYGQEAIEILLQVMENNR
DDLVVILAGYADRMENFFQSNPGFRSRIAHHIEFPDYSDEELFEIAGHMLDDQNYQMTPEAETALRAYIGLRRNQPHFAN
ARSIRNALDRARLRQANRLFTASSGPLDARALSTIAEEDIRASRVFKGG

CRYSTAL STRUCTURE OF THE AAA+ PROTEIN CBBX, NATIVE STRUCTURE

CRYSTAL STRUCTURE OF THE AAA+ PROTEIN CBBX, SELENOMETHIONINE STRUCTURE

ADENOSINE-5'-DIPHOSPHATE (ADP): MODELLED FROM P97 D2 (PDB 3CF3)