4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID

Probing the Conformational Mobility of the Active Site of Ascorbate Peroxidase

MRGSHHHHHHGSGKSYPTVSADYQKAVEKAKKKLRGFIAEKRCAPLMLRLAAMSAGTFDKGTKTGGPFGTIKHPAELAHS
ANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAVEVTGGPEVPFHPGREDKPEPPPEGRLPDATKGSDHLRDVFGKAM
GLTDQDIVALSGGHTIGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDKYAADED
AFFADYAEAHQKLSELGFADA

PREVIOUS REFINEMENT CYCLES WERE CARRIED OUT WITH REFMAC 5.0. THERE IS ONE DISORDERED REGION IN THE STRUCTURE, BETWEEN RESIDUES 65 AND 70. THIS REGION WAS MODELLED ON THE FO-FC MAP, CALCULATED WHEN THE OCCUPANCY OF RESIDUES WAS SET TO 0.