Catalytic domain of mouse 2',3'-cyclic nucleotide 3'- phosphodiesterase, with mutation H230S, crystallized with NADP

NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE
2'-MONOPHOSPHOADENOSINE 5'-DIPHOSPHORIBOSE

Crystallographic Analysis of the Reaction Cycle of 2',3'-Cyclic Nucleotide 3'-Phosphodiesterase, a Unique Member of the 2H Phosphoesterase Family

MULTILAYER MIRROR, CURVED TO FOCUS IN THE VERTICAL (R 400 M)

BENT SI (111) CRYSTAL, HORIZONTALLY FOCUSING

2', 3'-CYCLIC-NUCLEOTIDE 3'-PHOSPHODIESTERASE
C-TERMINAL CATALYTIC DOMAIN, RESIDUES 159-378

GGLEKDFLPLYFGWFLTKKSSETLRKAGQVFLEELGNHKAFKKELRHFISGDEPKEKLELVSYFGKRPPGVLSCTTKFCD
YGKAAGAEEYAQQEVVKRSYGKAFKLSISALFVTPKTAGAQVVLTDQELQLWPSDLDKPSASEGLPPGSRAHVTLGCAAD
VQPVQTGLDLLDILQQVKGGSQGEAVGELPRGKLYSLGKGRWMLSLTKKMEVKAIFTGYYG

250 UM PROTEIN WITH 10 MM 23-CNADP MIXED IN 0.5 PLUS 0.5 UL DROPS WITH 50 MM NA-FORMATE (1:2 PH3:PH5) AND 23% PEG 4000 IN 4 DEGC

CATALYTIC DOMAIN OF MOUSE 2',3'-CYCLIC NUCLEOTIDE 3'- PHOSPHODIESTERASE, COMPLEXED WITH CITRATE

CATALYTIC DOMAIN OF MOUSE 2',3'-CYCLIC NUCLEOTIDE 3'- PHOSPHODIESTERASE, COMPLEXED WITH SULFATE

CATALYTIC DOMAIN OF MOUSE 2',3'-CYCLIC NUCLEOTIDE 3'- PHOSPHODIESTERASE, SOAKED WITH 2',3'-CYCLIC NADP

CATALYTIC DOMAIN OF MOUSE 2',3'-CYCLIC NUCLEOTIDE 3'- PHOSPHODIESTERASE, SOAKED WITH GTP

CATALYTIC DOMAIN OF MOUSE 2',3'-CYCLIC NUCLEOTIDE 3'- PHOSPHODIESTERASE, SOAKED WITH 2',3'-CYCLIC AMP

CATALYTIC DOMAIN OF MOUSE 2',3'-CYCLIC NUCLEOTIDE 3 '- PHOSPHODIESTERASE, CRYSTALLIZED WITH 2'-AMPS

CATALYTIC DOMAIN OF MOUSE 2',3'-CYCLIC NUCLEOTIDE 3 '- PHOSPHODIESTERASE, WITH MUTATION H309S, CRYSTALLIZED WITH 2',3'-CYCLIC AMP

CATALYTIC DOMAIN OF MOUSE 2',3'-CYCLIC NUCLEOTIDE 3 '- PHOSPHODIESTERASE, WITH MUTATION H230S, CRYSTALLIZED WITH 2',3-(RP)-CYCLIC-AMPS

CATALYTIC DOMAIN OF MOUSE 2',3'-CYCLIC NUCLEOTIDE 3 '- PHOSPHODIESTERASE, WITH MUTATION V321A, CRYSTALLIZED WITH 2'-AMP

N-TERMINAL GLYCINE REMAINS FROM TEV-CLEAVAGE OF EXPRESSION TAG. RESIDUE NUMBERING IS ACCORDING TO CNPASE ISOFORM 2 (P16330-2). HISTIDINE 230 IS MUTATED TO SERINE.

HYDROGENS WERE INCLUDED IN RIDING POSITIONS. RESIDUES 158-160, 210-212 FROM CHAIN A, AND RESIDUES 158-160, 210-213 AND 294-296 WERE EXCLUDED FROM THE MODEL DUE TO INSUFFICIENT ELECTRON DENSITY