The I375W mutant of CsyB complexed with CoA-SH

Structural basis for the formation of acylalkylpyrones from two beta-ketoacyl units by the fungal type III polyketide synthase CsyB.

MGSSHHHHHHSSGLVPRGSHMIEPLPTEDIPKQSVSIVGIASRCAPHKLGADELEAIARRHYSSTPSLEKMLEINRKTRI
DHRYSVFSSDHEHWHRPTIPSFSECDSLFKEYGIPLASAASARAIQDWGGVPDEITHLVAVTCTNTAHPGFDSVLCRKLG
LKCNVRRVLLHGIGCGGGISAMRVAHELLLGSTQQGVPARALIVACEVPTVFARSELDIMDKTQDVNVAMCLFGDCAAAL
VLSNGIGHKASEQRPIWNILNCEPTQFDGTEDIAHFNVHDKGYHAIIDKRIPQLTGKCVPAGFQSLISSTPSLALEEKNY
VPSNYGWAVHPGGYAVLVAAQDALGLTADDLRASYDAYRDGGNTISTTIIRILEKLRDEHKHGSNQKDKLVLAAWGHGIT
LETAILTRPGSSSYLHA

100mM PIPES-NaOH, 6% PEG 4000, 250mM LiCl, 2mM CoA-SH, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K

CsyB, Type III polyketide synthase, Acyltransferase, TRANSFERASE

MIEPLPTEDIPKQSVSIVGIASRCAPHKLGADELEAIARRHYSSTPSLEKMLEINRKTRIDHRYSVFSSDHEHWHRPTIP
SFSECDSLFKEYGIPLASAASARAIQDWGGVPDEITHLVAVTCTNTAHPGFDSVLCRKLGLKCNVRRVLLHGIGCGGGIS
AMRVAHELLLGSTQQGVPARALIVACEVPTVFARSELDIMDKTQDVNVAMCLFGDCAAALVLSNGIGHKASEQRPIWNIL
NCEPTQFDGTEDIAHFNVHDKGYHAIIDKRIPQLTGKCVPAGFQSLISSTPSLALEEKNYVPSNYGWAVHPGGYAVLVAA
QDALGLTADDLRASYDAYRDGGNTISTTIIRILEKLRDEHKHGSNQKDKLVLAAIGHGITLETAILTRPGSSSYLHA