Crystal structure of antifreeze protein from an Antarctic sea ice bacterium Colwellia sp.

Hyperactive antifreeze protein from an Antarctic sea ice bacterium Colwellia sp. has a compound ice-binding site without repetitive sequences.

AGPYAVELGEAGTFTILSKSGITDVYPSTVTGNVGTSPITGAALLLNCDEVTGAMYTVDSAGPLPCSINSPYLLELAVSD
MGIAYNDAAGRVPADHTELGTGEIGGLTLEPGVYKWSSDVNISTDVTFNGTMDDVWIMQISGNLNQANAKRVTLTGGALA
KNIFWQVAGYTALGTYASFEGIVLSKTLISVNTGTTVNGRLLAQTAVTLQKNTINAPTEQYEEAPL

0.1M Tris-HCl, 0.2M sodium acetate, 30% polypolyethylene glycol 4000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Right-handed Beta-helix, antifreeze protein