Crystal Structure of pOPH S172A with octanoic acid

Structural insights into enzymatic degradation of oxidized polyvinyl alcohol

OPH, Oxidized PVA hydrolase, Beta-diketone hydrolase

AGAGADRTAATPAAANPAATEPVKWECPAGYEVKEGLNVDFPHKGMKRAFIVYPAKNVSGPAPVWVPMTGSVESTNDNLT
VARSGANSILADHGYTVIAPVRACANQDPNIRGERCNGPGSNGWNWNPWFEGRAADPSGEHWKNDEGPDSSFFVAMVQCV
GTKYKLDARRLFLGGIASGGTMTNRALLFRSNFWAGGLPISGEWYVTSDDGTPLSFDDARAAVAAAPTKIHQGRVGPYPL
PAKVGPLIVMTVWGGEKDLWNCTRPDGSRFLCADYRPSTQAGSNFFSAQPDVVHVACSSTHGHMWPQLNTQEFNRWALDT
LASHPKGSDPRSFKLTQPPEGYTCHVGPFTGLYASAWSHPQFEK

0.1M tri-sodium citrate, pH 5.6, 30% w/v Polyethylene Glycol 4000, 6% w/v n-Octyl-beta-D-glucoside, VAPOR DIFFUSION, SITTING DROP, temperature 295K

alpha/beta-hydrolase, oxi-polyvinyl alcohol hydrolase, HYDROLASE

GADRTAATPAAANPAATEPVKWECPAGYEVKEGLNVDFPHKGMKRAFIVYPAKNVSGPAPVWVPMTGSVESTNDNLTVAR
SGANSILADHGYTVIAPVRACANQDPNIRGERCNGPGSNGWNWNPWFEGRAADPSGEHWKNDEGPDSSFFVAMVQCVGTK
YKLDARRLFLGGISSGGTMTNRALLFRSNFWAGGLPISGEWYVTSDDGTPLSFDDARAAVAAAPTKIHQGRVGPYPLPAK
VGPLIVMTVWGGEKDLWNCTRPDGSRFLCADYRPSTQAGSNFFSAQPDVVHVACSSTHGHMWPQLNTQEFNRWALDTLAS
HPKGSDPRSFKLTQPPEGYTCHVGPFTGLY