Crystal structure of Saccharomyces cerevisiae Atg12-Atg5 conjugate bound to the N-terminal domain of Atg16

Structure of the Atg12-Atg5 conjugate reveals a platform for stimulating Atg8-PE conjugation

Cytoplasm to vacuole targeting protein 11, SAP18 homolog

MNDIKQLLWNGELNVLVSIDPSFLMKGSPREIAVLRIRVPRETYLVNYMPLIWNKIKSFLSFDPLTDSEKYFWFEHNKTP
IPWNYPVGVLFDCLAGKSATFTTSFENQVKDVLTFLRIHLVMGDSLPPTIIPIASSKTQAEKFWFHQWKQVCFILNGSSK
AIMSLSVNEARKFWGSVITRNFQDFIEISNKISSSRPRHIPLIIQTSRTSGTFRISQPTISMTGVNPTLKDIEGDILDVK
EGINGNDVMVICQGIEIPWHMLLYDLYSKLRSFDGFLYITLVPI

GAHMNIQKIQIKFQPIGSIGQLKPSVCKISMSQSFAMVILFLKRRLKMDHVYCYINNSFAPSPQQNIGELWMQFKTNDEL
IVSYCASVAFG

APG5, ATG5, Atg5(amino acids 1-284), P2601, YPL149W

APG15, APG16, ATG16, Atg16(amino acids 1-46), CVT11, SAP18, YM8520.08C, YMR159C

APG12, ATG12, Atg12(amino acids 100-186), YBR1506, YBR217W

12% PEG10000, 0.5M potassium thiocyanate, 0.1M ADA, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K

Ubiquitin fold, E3-like, Atg3 binding, Isopeptide bond between Atg12 Gly186 and Atg5 Lys149, LIGASE

NIQKIQIKFQPIGSIGQLKPSVCKISMSQSFAMVILFLKRRLKMDHVYCYINNSFAPSPQQNIGELWMQFKTNDELIVSY
CASVAFG