The crystal structure of Xenopus Glucokinase and Glucokinase Regulatory Protein complex

Molecular basis for the role of glucokinase regulatory protein as the allosteric switch for glucokinase

Hexokinase IV inhibitor, Glucokinase regulator

METFDQNEVDQILSEFHLQEEDLHVLMCRMQAEMERGLHLETNEEASVKMLPTYVRSTPDGSEVGDFLALDLGGTNFRVM
LVKVGEDLEGQWKVETKHKMYSIPVDAMTGTAEMLFDYIAECISDYLDQQNMKHKKLPLGFTFSFPVRHEDIDKGILLNW
TKGFKASGAEGNNVVGLLRDAIKRRGDFEMDVVAMVNDTVATMISCYYEDHHCEVGLIVGTGCNACYMEEMSNVELVEGE
EGRMCVNTEWGAFGDTGELEDFRLEYDRVVDEASLNPGQQLYEKMIGGKYMGELVRLVLIKMVNENLLFGGESSEKLKTR
GAFETQFVSQIEADTSDFKQTLNILRTLGVQATIGDCHAVRLACESVSTRAAIMCSAGLAGILNRMRQSRREELLRITVG
VDGSVYKLHPSFKDKFHATVLKLTSGCEITFIQSEEGSGRGAALISAVAYKMAVMIGH

MRGTRKYQHVIETPDPGKWELAGYEESLPISEKSNPMTRELDKADPSQLVQLLRDCDAEIFQEEDENLIHYHRLYSESVL
KTMGDVAKRVQEVLKNPDDSLVVLSGCGTSGRLALLLANSFNGLLKGLHKTPCYCYIMSGGDRSIVTSQESSEDNPQLGA
QELEKVCEGKKNVLFIGISCGLSAPFIAGQLDFCMRHLDVYLPVLVGFNPVSMARNERIEGWHSSFRQVAERLQTLHDSQ
KGFILNPAVGPEGVSGSSRMKGGSATKILLETLLLVAHKAESNVPVTEKCLLEILRTYERAHKVTYSQSKKIAALMKQTA
TSLQKKGHLYILGWGTLGLVGIMDAVECVPTYQADWRDVRGFITGGYHSIENKEGDLSSLGPQFSISHEDFVKNVLPSVS
ETDTVLLIFTLDDDLNQIEKLVALVKEKTSNIQVICHATAGQYLPNSLKKTIPSIIGLTWPILFLEYEGAFIQKFQRELS
TKWILDTVTSGAYTLRGKIFRNFMVDFKINNSKLFHRATSVLQRLTGQSQQRCTEVLLQSIYGEQTLSEQIRNTTIAGHV
EAAASQDKVLPVAIVSLLRSCTIQDSRSRINSSLSIRSAIESSMNVPGRKRGAEDSESR

0.005M MgSO4, 0.05M MES, 5% PEG4000, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K

THIS SEUQENCE IS ACCORDING TO REFERENCE SEQUENCE NM_001089807.

aba sandwich, typical hexokanse fold, kinase, sugar binding, TRANSFERASE-TRANSFERASE INHIBITOR complex

MRGTRKYQHVIETPDPGKWELAGYEESLPISEKSNPMTRELDKADPSQLVQLLRDCDAEIFQEEDENLIHYHRLYSESVL
KTMGDVAKRVQEVLKNPDDSLVVLSGCGTSGRLALLLANSFNGLLKGLHKTPCYCYIMSGGDRSIVTSQESSEDNPQLGA
QELEKVCEGKKNVLFIGISCGLSAPFIAGQLDFCMRHLDVYLPVLVGFNPVSMARNERIEGWHSSFRQVAERLQTLHDSQ
KGFILNPAVGPEGVSGSSRMKGGSATKILLETLLLVAHKAESNVPVTEKCLLEILRTYERAHKVTYSQSKKIAALMKQTA
TSLQKKGHLYILGWGTLGLVGIMDAVECVPTYQADWRDVRGFITGGYHSIENKEGDLSSLGPQFSISHEDFVKNVLPSVS
ETDTVLLIFTLDDDLNQIEKLVALVKEKTSNIQVICHATAGQYLPNSLKKTIPSIIGLTWPILFLEYEGAFIQKFQRELS
TKWILDTVTSGAYTLRGKIFRNFMVDFKINNSKLFHRATSVLQRLTGQSHQRCTEVLLQSIYGEQTLSEQIRNTTIAGHV
EAAASQDKVLPVAIVSLLRSCTIQDSRSRINSSLSIRSAIESSMNVPGRKRGAEDSESR