Crystal structure of Kluyveromyces marxianus Atg7NTD-Atg10 complex

Noncanonical recognition and UBL loading of distinct E2s by autophagy-essential Atg7.

Numerical link type Si(111) double crystal monochromator

GPHMMVSDLKFAPSFQSFVDSSFFHELSRLKLDIFKLDSDEKALYTQLDLNQFTSNVLAISLRDDSFQKPDNDEHNIILK
GYLLNFNTIELFKNCNKIQFIKEKGQELLQRGLENDLNEIISFYMISFADLKKYKFYYWICMPSFQSDGATYQIISSKVI
ASDSDISVSFIKQNVIIACVISGVIQKATPDNLKVCEKVVFKDFSHLKDIPSAVTKNILTVWSKLSPRETYTICFLRSDE
SSFEAEIIINNGNNPSLKVSGWEKNGLGKLAPKSIDLSSLMDP

GPLGSMLTLPEYNEQIPNVRSLLTKWAKVERIQDVQDGLQLDVRLKTDTLLELHIYYDHVYHVPSIKFRLWSLDTEEDIS
SLRLLTLSDSELRSILNLGTFSVTLSTDMEMKSVYYYINNCDTDANVGSDVEHYLTRWISLYIRIFDLNFVP

0.8M sodium phosphate, 0.7M potassium phosphate, 0.2M lithium sulfate, 0.1M CHES, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K

SEQUENCE DATABASE REFERENCE FOR EACH CHAIN DOES NOT CURRENTLY EXIST.

ubiquitin conjugation, E1-E2 complex, Ligase