Crystal structure of beta-glucosidase from termite Neotermes koshunensis in complex with Bis-Tris

High-resolution structures of Neotermes koshunensis beta-glucosidase mutants provide insights into the catalytic mechanism and the synthesis of glucoconjugates

Vertically Collimating Premirror, Toroidal Focusing Mirror

LN2-Cooled Fixed-Exit Double Crystal Si(111) Monochromator

MDVASSDTVYTFPDEFKLGAATASYQIEGAWDENGKGPNIWDTLTHEHPDYVVDGATGDIADDSYHLYKEDVKILKELGA
QVYRFSISWARVLPEGHDNIVNQDGIDYYNNLINELLANGIEPMVTMYHWDLPQALQDLGGWPNLVLAKYSENYARVLFK
NFGDRVKLWLTFNEPLTFMDGYASEIGMAPSINTPGIGDYLAAHTVIHAHARIYHLYDQEFRAEQGGKVGISLNINWCEP
ATNSAEDRASCENYQQFNLGLYAHPIFTEEGDYPAVLKDRVSRNSADEGYTDSRLPQFTAEEVEYIRGTHDFLGINFYTA
LLGKSGVEGYEPSRYRDSGVILTQDAAWPISASSWLKVVPWGFRKELNWIKNEYNNPPVFITENGFSDYGGLNDTGRVHY
YTEHLKEMLKAIHEDGVNVIGYTAWSLMDNFEWLRGYSEKFGIYAVDFEDPARPRIPKESAKVLAEIMNTRKIPERFRDL
EHHHHHH

0.1 M Bis-Tris, 18-21%(w/v) PEG 3350, 0.1-0.25 M MgCl2, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

CELLULASES, GLYCOSYL HYDROLASE, HYDROLASE

DVASSDTVYTFPDEFKLGAATASYQIEGAWDENGKGPNIWDTLTHEHPDYVVDGATGDIADDSYHLYKEDVKILKELGAQ
VYRFSISWARVLPEGHDNIVNQDGIDYYNNLINELLANGIEPMVTMYHWDLPQALQDLGGWPNLVLAKYSENYARVLFKN
FGDRVKLWLTFNEPLTFMDGYASEIGMAPSINTPGIGDYLAAHTVIHAHARIYHLYDQEFRAEQGGKVGISLNINWCEPA
TNSAEDRASCENYQQFNLGLYAHPIFTEEGDYPAVLKDRVSRNSADEGYTDSRLPQFTAEEVEYIRGTHDFLGINFYTAL
LGKSGVEGYEPSRYRDSGVILTQDAAWPISASSWLKVVPWGFRKELNWIKNEYNNPPVFITENGFSDYGGLNDTGRVHYY
TEHLKEMLKAIHEDGVNVIGYTAWSLMDNFEWLRGYSEKFGIYAVDFEDPARPRIPKESAKVLAEIMNTRKIPERFRD