Crystal structure of -35 promoter binding domain of SigD of Mycobacterium tuberculosis
Mycobacterium tuberculosis RsdA provides a conformational rationale for selective regulation of sigma-factor activity by proteolysis
(MSE)GSSHHHHHHSQDP(MSE)AIEADSVTR(MSE)NELLEILPAKQREILILRVVVGLSAEETAAAVGSTTGAVRVAQ HRALQRLKDEIVAAGDYA
MGSSHHHHHHSQDPMAIEADSVTRMNELLEILPAKQREILILRVVVGLSAEETAAAVGSTTGAVRVAQHRALQRLKDEIV AAGDYA
1.0-1.3M ammonium sulfate, 0.1M Tris-Cl, 5% 2-Methyl-2,4-pentanediol(MPD), 3% Glycerol, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 300K
Transcription regulation, Promoter, Anti-sigma factors, TRANSCRIPTION