Crystal Structure of the CFA/I Enterotoxigenic E. coli adhesin CfaE mutant G168D

Tight Conformational Coupling between the Domains of the Enterotoxigenic Escherichia coli Fimbrial Adhesin CfaE Regulates Binding State Transition.

dscCfaE, Colonization factor antigen I subunit E

ADKNPGSENMTNTIGPHDRGGSSPIYNILNSYLTAYNGSHHLYDRMSFLCLSSQNTLNGACPSSDAPGTATIDGETNITL
QFTEKRSLIKRELQIKGYKQFLFKNANCPSKLALNSSHFQCNREQASGATLSLYIPAGELNKLPFDGVWNAVLKLNVKRR
YDTTYGTYTINITVNLTDKGNIQIWLPQFKSNARVDLNLRPTGGGTYIGRNSVDMCFYDGYSTNSSSLEIRFQDDNSKSD
GKFYLKKINDDSKELVYTLSLLLAGKNLTPTNGQALNINTASLETNWNRITAVTMPEISVPVLCWPGRLQLDAKVKNPEA
GQYMGNIKITFTPSSQTLDNKQVEKNITVTASVDPV

0.1 M sodium citrate, pH 5.8, 1.5 M sodium chloride, 18% PEG4000, 50 mM guanidine chloride, VAPOR DIFFUSION, HANGING DROP, temperature 288K

The wild type protein of the CFA/I enterotoxigenic E. coli adhesin CfaE

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : RESIDUAL ONLY

Ig fold, CFA/I ETEC adhesin, CELL ADHESION

ADKNPGSENMTNTIGPHDRGGSSPIYNILNSYLTAYNGSHHLYDRMSFLCLSSQNTLNGACPSSDAPGTATIDGETNITL
QFTEKRSLIKRELQIKGYKQFLFKNANCPSKLALNSSHFQCNREQASGATLSLYIPAGELNKLPFGGVWNAVLKLNVKRR
YDTTYGTYTINITVNLTDKGNIQIWLPQFKSNARVDLNLRPTGGGTYIGRNSVDMCFYDGYSTNSSSLEIRFQDDNSKSD
GKFYLKKINDDSKELVYTLSLLLAGKNLTPTNGQALNINTASLETNWNRITAVTMPEISVPVLCWPGRLQLDAKVKNPEA
GQYMGNIKITFTPSSQTL