Crystal Structure of Phosphoglycerate Kinase from Bacillus Anthracis

Center for Structural Genomics of Infectious Diseases (CSGID)

Crystal structures of putative phosphoglycerate kinases from B. anthracis and C. jejuni.

(MSE)NKKSIRDVDLKGKRVFCRVDFNVP(MSE)KEGKITDETRIRAALPTIQYLVEQGAKVILASHLGRPKGQAVEELR
LTPVAARLGELLGKDVKKADEAFGPVAQE(MSE)VAA(MSE)NEGDVLVLENVRFYAGEEKNDAELAKEFAALADIFVND
AFGAAHRAHASTAGIADYLPAVSGLL(MSE)EKELEVLGKALSNPERPFAAIIGGAKVKDKIGLIRHLLDKVDNLIIGGG
LAYTFVKALGHEIGLSLCEDDKIELAKEF(MSE)QLAKEKGVNFY(MSE)PVDVVITEEFSETATTKIVGIDSIPSNWEG
VDIGPKTREIYADVIKNSKLVVWNGP(MSE)GVFE(MSE)TPFAEGTKAVGQALADAEGTYSVIGGGDSAAAVEKFG
(MSE)ADK(MSE)SHISTGGGASLEF(MSE)EGKELPGVVCLNDK

MNKKSIRDVDLKGKRVFCRVDFNVPMKEGKITDETRIRAALPTIQYLVEQGAKVILASHLGRPKGQAVEELRLTPVAARL
GELLGKDVKKADEAFGPVAQEMVAAMNEGDVLVLENVRFYAGEEKNDAELAKEFAALADIFVNDAFGAAHRAHASTAGIA
DYLPAVSGLLMEKELEVLGKALSNPERPFAAIIGGAKVKDKIGLIRHLLDKVDNLIIGGGLAYTFVKALGHEIGLSLCED
DKIELAKEFMQLAKEKGVNFYMPVDVVITEEFSETATTKIVGIDSIPSNWEGVDIGPKTREIYADVIKNSKLVVWNGPMG
VFEMTPFAEGTKAVGQALADAEGTYSVIGGGDSAAAVEKFGMADKMSHISTGGGASLEFMEGKELPGVVCLNDK

25% PEG 3350, 0.2M NaCl, 0.1M Bis-Tris, 2% Hexandiol, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Center for Structural Genomics of Infectious Diseases

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

Anthrax, Structural Genomics, Center for Structural Genomics of Infectious Diseases, CSGID, Rossmann fold, phosphoglycerate kinase, phosphoglycerate, Phosphorylation, TRANSFERASE

MNKKSIRDVDLKGKRVFCRVDFNVPMKEGKITDETRIRAALPTIQYLVEQGAKVILASHLGRPKGQAVEELRLTPVAARL
GELLGKDVKKADEAFGPVAQEMVAAMNEGDVLVLENVRFYAGEEKNDAELAKEFAALADIFVNDAFGAAHRAHASTAGIA
DYLPAVSGLLMEKELEVLGKALSNPERPFTAIIGGAKVKDKIGLIRHLLDKVDNLIIGGGLAYTFVKALGHEIGLSLCED
DKIELAKEFMQLAKEKGVNFYMPVDVVITEEFSETATTKIVGIDSIPSNWEGVDIGPKTREIYADVIKNSKLVVWNGPMG
VFEMTPFAEGTKAVGQALADAEGTYSVIGGGDSAAAVEKFGMADKMSHISTGGGASLEFMEGKELPGVVCLNDK