ATP synthase C10 ring in proton-unlocked conformation at PH 5.5

Structure of the c(10) ring of the yeast mitochondrial ATP synthase in the open conformation.

68% MPD, 8% PROPOLYENE GLYCOL, 0.3M NACL, 0.1M MALONATE PH 7.0, 2MM MGSO4, 50 MM MES PH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K. SOAKING: 68% MPD, 8% PROPYLENE GLYCOL, 0.4M NACL, 2MM MGCL2, 50MM MES PH 5.5, FOR 12 HOURS AT 294K.

HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT

F1FO ATP synthase, proton pore, C10 ring, membrane protein