Structure and Catalytic Mechanism of the Vitamin B3 Degradative Enzyme Maleamate Amidohydrolase from Bordetalla bronchiseptica RB50

Structure and Catalytic Mechanism of Nicotinate (Vitamin B(3)) Degradative Enzyme Maleamate Amidohydrolase from Bordetella bronchiseptica RB50.

GSDKIHHHHHHSSGENLYFQGHMGNDLGSYERQGFGAALPLKAPYGLLIVDFVNGFADPAQFGGGNIAAAIETTRTVLAA
ARERGWAVAHSRIVYADDDADGNIFSIKVPGMLTLKEHAPASAIVPQLAPQAGEYVVRKSTPSAFYGTMLAAWLAQRGVQ
TLLVAGATTSG(CSO)VRASVVDAMSAGFRPLVLSDCVGDRALGPHEANLFDMRQKYAAVMTHDEALAKTKGLEHHHHHH

GSDKIHHHHHHSSGENLYFQGHMGNDLGSYERQGFGAALPLKAPYGLLIVDFVNGFADPAQFGGGNIAAAIETTRTVLAA
ARERGWAVAHSRIVYADDDADGNIFSIKVPGMLTLKEHAPASAIVPQLAPQAGEYVVRKSTPSAFYGTMLAAWLAQRGVQ
TLLVAGATTSGCVRASVVDAMSAGFRPLVLSDCVGDRALGPHEANLFDMRQKYAAVMTHDEALAKTKGLEHHHHHH

0.2 M ammonium acetate, 0.1 M sodium cacodylate, 30% PEG-8000, pH 6.50, VAPOR DIFFUSION, HANGING DROP, temperature 295K

PDB ENTRY 1NBA MODIFIED TO TARGET SEQUENCE USING PHYRE SERVER

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

MGNDLGSYERQGFGAALPLKAPYGLLIVDFVNGFADPAQFGGGNIAAAIETTRTVLAAARERGWAVAHSRIVYADDDADG
NIFSIKVPGMLTLKEHAPASAIVPQLAPQAGEYVVRKSTPSAFYGTMLAAWLAQRGVQTLLVAGATTSGCVRASVVDAMS
AGFRPLVLSDCVGDRALGPHEANLFDMRQKYAAVMTHDEALAKTK