Mouse Lin28A in complex with let-7d microRNA pre-element

Molecular Basis for Interaction of let-7 MicroRNAs with Lin28.

RNA (5'-R(*GP*GP*GP*CP*AP*GP*GP*GP*AP*UP*UP*UP*UP*GP*CP*CP*CP*GP*GP*AP*G)-3')

AADEPQLLHGAGICKWFNVRMGFGFLSMTARAGVALDPPVDVFVHQSKLHMEGFRSLKEGEAVEFTFKKSAKGLESIRVT
GPGGVFCIGSERRPKGGDRCYNCGGLDHHAKECKLPPQPKKCHFCQSINHMVASCPLKAQQGPSSQGK

0.6M NaH2PO4, 1.4M K2HPO4, pH 7, vapor diffusion, temperature 298K

In the crystal forms, the authors observe a domain swap in which the Lin28 CSD interacts with the loop of one microRNA molecule, and the CCHCx2 interacts with the GGAG of a second microRNA.

microRNA biogenesis, Protein-RNA complex, pre-element, CCHC zinc knuckle, cold shock domain, RNA BINDING PROTEIN-RNA complex

AADEPQLLHGAGICKWFNVRMGFGFLSMTARAGVALDPPVDVFVHQSKLHMEGFRSLKEGEAVEFTFKKSAKGLESIRVT
GPGGVFCIGSERRPKGKNMQKRRSKGDRCYNCGGLDHHAKECKLPPQPKKCHFCQSINHMVASCPLKAQQGPSSQGK