Processed Aspartate Decarboxylase Mutant with Asn72 mutated to Ala

Structure of Escherichia coli aspartate alpha-decarboxylase Asn72Ala: probing the role of Asn72 in pyruvoyl cofactor formation

horizontally focusing optimized for 1.488 and 0.98A

SCAIDQDFLDAAGILENEAIDIWNVTNGKRFSTYAIAAERGSRIISVAGAAAHCASVGDIVIIASFVTMPDEEARTWRPN
VAYFEGDNEMKRTAKAIPVQVA

1.6-2.4M ammonium sulphate, 0.1M citric acid, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

An alternative point mutant of the same protiein

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

SCAIDQDFLDAAGILENEAIDIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWRPN
VAYFEGDNEMKRTAKAIPVQVA