1.5 Angstrom Resolution Crystal Structure of K135M Mutant of Transaldolase B (TalA) from Francisella tularensis in Complex with Sedoheptulose 7-phosphate.

Center for Structural Genomics of Infectious Diseases (CSGID)

4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID

7-O-PHOSPHONO-D-ALTRO-HEPT-2-ULOSE; SEDOHEPTULOSE 7-PHOSPHATE

Adherence to Burgi-Dunitz stereochemical principles requires significant structural rearrangements in Schiff-base formation: insights from transaldolase complexes.

MHHHHHHSSGVDLGTENLYFQSNAMQKSVLEQLKQVTMVVADTGDFELIKKYKPVDATTNPSLILKAVKEQKYSNLVAET
ISKVKANNPDLNSDDLVKEIAIEILVSFGIKILDVIEGKVSSEVDARVSFNSATTIDYAKRIIARYESNGIPKDRVLIMI
AATWEGIKAAKLLQKEGINCNLTLIFDKAQAKACAEAGVYLVSPFVGRITDWQMQQNNLKTFPAIADDDGVNSVKAIYKL
YKSHGFKTIVMGASFRNVEQVIALAGCDALTISPVLLEELKNRDEHLEVKLTKNDDVVTQSPQISEADFRWLMNENAMAT
HKLAEGIRLFTKDTIELENIIKQNL

Francisella tularensis subsp. tularensis

Protein:9.4mG/mL, 0.5M Sodium chloride, 0.1M TRIS-HCl (pH 8.3), 0.015M Sedoheptulose 7-phosphate; Screen: PEG's (B6), 0.1M HEPES, 25% (w/v) PEG2000 MME., VAPOR DIFFUSION, SITTING DROP, temperature 295K

Center for Structural Genomics of Infectious Diseases

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

Structural Genomics, Center for Structural Genomics of Infectious Diseases, CSGID, Alpha-Beta Barrel/TIM Barrel, sedoheptulose-7-phosphate:D-glyceraldehyde-3-phosphate glyceronetransferase activity, TRANSFERASE

MQKSVLEQLKQVTMVVADTGDFELIKKYKPVDATTNPSLILKAVKEQKYSNLVAETISKVKANNPDLNSDDLVKEIAIEI
LVSFGIKILDVIEGKVSSEVDARVSFNSATTIDYAKRIIARYESNGIPKDRVLIKIAATWEGIKAAKLLQKEGINCNLTL
IFDKAQAKACAEAGVYLVSPFVGRITDWQMQQNNLKTFPAIADDDGVNSVKAIYKLYKSHGFKTIVMGASFRNVEQVIAL
AGCDALTISPVLLEELKNRDEHLEVKLTKNDDVVTQSPQISEADFRWLMNENAMATHKLAEGIRLFTKDTIELENIIKQN
L