Crystal structure of the cap free human translation initiation factor eIF4E

Structural insights into the allosteric effects of 4EBP1 on the eukaryotic translation initiation factor eIF4E.

Eukaryotic translation initiation factor 4E

eIF-4E, eIF4E, eIF-4F 25 kDa subunit, mRNA cap-binding protein

MATVEPETTPTPNPPTTEEEKTESNQEVANPEHYIKHPLQNRWALWFFKNDKSKTWQANLRLISKFDTVEDFWALYNHIQ
LSSNLMPGCDYSLFKDGIEPMWEDEKNKRGGRWLITLNKQQRRSDLDRFWLETLLCLIGESFDDYSDDVCGAVVNVRAKG
DKIAIWTTECENREAVTHIGRVYKERLGLPPKIVIGYQSHADTATKSGSTTKNRFVV

20% PEG-3350, 0.2 M diammonium citrate, vapor diffusion, temperature 289K

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. U VALUES: WITH TLS ADDED. Significant difference density is observed around gaps in protein chains C (residues 119-122) and D (residues 118-125) but is not interpretable in terms of peptide geometry. The program COOT and the MOLPROBITY server were also used in the course of model refinement.

eIF4E, Translation, mRNA export, Structural Genomics, Structural Genomics Consortium, SGC