The use of trimethylamine N-oxide as a primary precipitating agent and related methylamine osmolytes as cryoprotective agents for macromolecular crystallography.
Protein: 10 mg/mL in 50 mM CAPS pH 11; Well: 1.2 M TMAO, 0.1 M malate, pH 5.5 , VAPOR DIFFUSION, SITTING DROP, temperature 298K
TMAO-grown orthorhombic trypsin (bovine)
Orthorhombic trypsin (bovine) in the presence of sarcosine
Orthorhombic trypsin (bovine) in the presence of betaine
TMAO-grown trypsin (bovine)-previously unreported tetragonal crystal form
Tetragonal thermolysin in the presence of TMAO
Tetragonal thermolysin in the presence of sarcosine
Tetragonal thermolysin in the presence of betaine
HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS