Crystal Structure of Human Mre11: Understanding Tumorigenic Mutations

Double-strand break repair protein MRE11A

Meiotic recombination 11 homolog 1, MRE11 homolog 1, Meiotic recombination 11 homolog A, MRE11 homolog A

(MSE)GSSHHHHHHSSGLVPRGSH(MSE)STADALDDENTFKILVATDIHLGF(MSE)EKDAVRGNDTFVTLDEILRLAQ
ENEVDFILLGGDLFHENKPSRKTLHTCLELLRKYC(MSE)GDRPVQFEILSDQSVNFGFSKFPWVNYQDGNLNISIPVFS
IHGNHDDPTGADALCALDILSCAGFVNHFGRS(MSE)SVEKIDISPVLLQKGSTKIALYGLGSIPDERLYR(MSE)FVNK
KVT(MSE)LRPKEDENSWFNLFVIHQNRSKHGSTNFIPEQFLDDFIDLVIWGHEHECKIAPTKNEQQLFYISQPGSSVVT
SLSPGEAVKKHVGLLRIKGRK(MSE)N(MSE)HKIPLHTVRQFF(MSE)EDIVLANHPDIFNPDNPKVTQAIQSFCLEKI
EE(MSE)LENAERERLGNSHQPEKPLVRLRVDYSGGFEPFSVLRFSQKFVDRVANPKDIIHFFRHREQKEKTGEE

MGSSHHHHHHSSGLVPRGSHMSTADALDDENTFKILVATDIHLGFMEKDAVRGNDTFVTLDEILRLAQENEVDFILLGGD
LFHENKPSRKTLHTCLELLRKYCMGDRPVQFEILSDQSVNFGFSKFPWVNYQDGNLNISIPVFSIHGNHDDPTGADALCA
LDILSCAGFVNHFGRSMSVEKIDISPVLLQKGSTKIALYGLGSIPDERLYRMFVNKKVTMLRPKEDENSWFNLFVIHQNR
SKHGSTNFIPEQFLDDFIDLVIWGHEHECKIAPTKNEQQLFYISQPGSSVVTSLSPGEAVKKHVGLLRIKGRKMNMHKIP
LHTVRQFFMEDIVLANHPDIFNPDNPKVTQAIQSFCLEKIEEMLENAERERLGNSHQPEKPLVRLRVDYSGGFEPFSVLR
FSQKFVDRVANPKDIIHFFRHREQKEKTGEE

14% PEG 3350, 0.1M Bicne, 0.5mM manganese chloride, 2mM Dithiothreitol, 4mM Glutathione, pH 8.8, VAPOR DIFFUSION, HANGING DROP, temperature 291K

DNA repair, MRN complex, metallophosphatase, Exonuclease, Endonuclease, Rad50, Nbs1, HYDROLASE

chain A and (resseq 8:95 or resseq 116:400 )

chain B and (resseq 8:95 or resseq 116:400 )

chain C and (resseq 8:95 or resseq 116:400 )

chain D and (resseq 8:95 or resseq 116:400 )

MSTADALDDENTFKILVATDIHLGFMEKDAVRGNDTFVTLDEILRLAQENEVDFILLGGDLFHENKPSRKTLHTCLELLR
KYCMGDRPVQFEILSDQSVNFGFSKFPWVNYQDGNLNISIPVFSIHGNHDDPTGADALCALDILSCAGFVNHFGRSMSVE
KIDISPVLLQKGSTKIALYGLGSIPDERLYRMFVNKKVTMLRPKEDENSWFNLFVIHQNRSKHGSTNFIPEQFLDDFIDL
VIWGHEHECKIAPTKNEQQLFYISQPGSSVVTSLSPGEAVKKHVGLLRIKGRKMNMHKIPLHTVRQFFMEDIVLANHPDI
FNPDNPKVTQAIQSFCLEKIEEMLENAERERLGNSHQPEKPLVRLRVDYSGGFEPFSVLRFSQKFVDRVANPKDIIHFFR
HREQKEKTGEE