Crystal structure of FlhF in complex with its activator

Structural basis for the molecular evolution of SRP-GTPase activation by protein.

MGHHHHHHSSPKIEERTYPPQIPAQQELGDFSAYQSVLPEPLRKAEKLLQETGIKESTKTNTLKKLLRFSVEAGGLTEEN
VVGKLQEILCDMLPSADKWQEPIHSKYIVLFGSTGAGKTTTLAKLAAISMLEKHKKIAFITTDTYRIAAVEQLKTYAELL
QAPLEVCYTKEEFQQAKELFSEYDHVFVDTAGRNFKDPQYIDELKETIPFESSIQSFLVLSATAKYEDMKHIVKRFSSVP
VNQYIFTKIDETTSLGSVFNILAESKIGVGFMTNGQNVPEDIQTVSPLGFVRMLCR

0.1 M Bis-Tris, 1.8 M Ammoniumsulfate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K

SRP GTPASE, FLAGELLUM, PROTEIN TRANSPORT, BIOSYNTHETIC PROTEIN, GTPase ACTIVATING PROTEIN, Type 3 secretion system, GTP-binding protein

SSPKIEERTYPPQIPAQQELGDFSAYQSVLPEPLRKAEKLLQETGIKESTKTNTLKKLLRFSVEAGGLTEENVVGKLQEI
LCDMLPSADKWQEPIHSKYIVLFGSTGAGKTTTLAKLAAISMLEKHKKIAFITTDTYRIAAVEQLKTYAELLQAPLEVCY
TKEEFQQAKELFSEYDHVFVDTAGRNFKDPQYIDELKETIPFESSIQSFLVLSATAKYEDMKHIVKRFSSVPVNQYIFTK
IDETTSLGSVFNILAESKIGVGFMTNGQNVPEDIQTVSPLGFVRMLCR