Engineered high-affinity halide-binding protein derived from YFP: fluoride complex

{(4Z)-2-(aminomethyl)-4-[(4-hydroxyphenyl)methylidene]-5-oxo-4,5-dihydro-1H-imidazol-1-yl}acetic acid

Determination of engineered chloride-binding site structures in fluorescent proteins reveals principles of halide recognition

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTF(CR2)VQCFARYPDH
MRQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQ
KNGIKVNFKIRHNIEDGSVQLADHYAQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELY
KGGSNDYKDDDDKGGSHHHHHH

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMR
QHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
GIKVNFKIRHNIEDGSVQLADHYAQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKG
GSNDYKDDDDKGGSHHHHHH

17% PEG3000, 100 mM sodium acetate, 90 mM magnesium chloride, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 290K

Engineered high-affinity halide-binding protein derived from YFP: halide-free

Engineered high-affinity halide-binding protein derived from YFP: chloride complex

Engineered high-affinity halide-binding protein derived from YFP: bromide complex

Engineered high-affinity halide-binding protein derived from YFP: iodide complex

Engineered low-affinity halide-binding protein derived from YFP: halide-free

Engineered low-affinity halide-binding protein derived from YFP: chloride complex

Engineered low-affinity halide-binding protein derived from YFP: fluoride complex

Engineered low-affinity halide-binding protein derived from YFP: iodide complex

Engineered low-affinity halide-binding protein derived from YFP: bromide complex

Engineered medium-affinity halide-binding protein derived from YFP: halide-free

Engineered medium-affinity halide-binding protein derived from YFP: iodide complex

Engineered medium-affinity halide-binding protein derived from YFP: fluoride complex

Engineered medium-affinity halide-binding protein derived from YFP: chloride complex

Engineered medium-affinity halide-binding protein derived from YFP: bromide complex

UNP RESIDUE SER65 UNDERWENT MUTATION TO GLY. GLY65, TYR66, AND GLY67 CIRCULARIZED INTO ONE CHROMOPHORE (CR2).

beta barrel, luminescent protein, yellow fluorescent protein, imaging reagent, HALIDE BINDING PROTEIN

MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQ
HDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK