Crystal structure of aprataxin ortholog Hnt3 in complex with DNA and AMP

Crystal structures of aprataxin ortholog Hnt3 reveal the mechanism for reversal of 5'-adenylated DNA

DNA (5'-D(*TP*AP*TP*TP*CP*CP*GP*AP*TP*AP*GP*TP*GP*AP*C)-3')

DNA (5'-D(*GP*TP*CP*AP*CP*TP*AP*TP*CP*GP*GP*AP*AP*TP*GP*AP*G)-3')

GSHMSFRDNLKVYIESPESYKNVIYYDDDVVLVRDMFPKSKMHLLLMTRDPHLTHVHPLEIMMKHRSLVEKLVSYVQGDL
SGLIFDEARNCLSQQLTNEALANYIKVGFHAGPSMNNLHLHIMTLDHVSPSLKNSAHYISFTSPFFVKIDTPTSNLPTRG
TLTSLFQEDLKCWRCGETFGRHFTKLKAHLQEEYDDWLDKSVSM

1.6M Ammonium Sulfate, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

DNA oligonucleotides were synthesized from Shanghai Sangon Biotechnology Company (Shanghai, China)

HIT domain, Zinc Finger, DNA deadenylase, DNA binding, AMP binding, HYDROLASE-DNA complex

SFRDNLKVYIESPESYKNVIYYDDDVVLVRDMFPKSKMHLLLMTRDPHLTHVHPLEIMMKHRSLVEKLVSYVQGDLSGLI
FDEARNCLSQQLTNEALCNYIKVGFHAGPSMNNLHLHIMTLDHVSPSLKNSAHYISFTSPFFVKIDTPTSNLPTRGTLTS
LFQEDLKCWRCGETFGRHFTKLKAHLQEEYDDWLDKSVSM