Crystal structure of fluorophore-labeled beta-lactamase PenP in complex with cephaloridine

5-METHYL-2-[2-OXO-1-(2-THIOPHEN-2-YL-ACETYLAMINO)-ETHYL]-3,6-DIHYDRO-2H-[1,3]THIAZINE-4-CARBOXYLIC ACID

Increased structural flexibility at the active site of a fluorophore-conjugated beta-lactamase distinctively impacts its binding toward diverse cephalosporin antibiotics

MKTEMKDDFAKLEEQFDAKLGIFALDTGTNRTVAYRPDERFAFASTIKALTVGVLLQQKSIEDLNQRITYTRDDLVNYNP
ITEKHVDTGMTLKELADASLRYSDNAAQNLILKQIGGPESLKKELRKIGDEVTNPERFCPELNEVNPGETQDTSTARALV
TSLRAFALEDKLPSEKRELLIDWMKRNTTGDALIRAGVPDGWEVADKTGAASYGTRNDIAIIWPPKGDPVVLAVLSSRDK
KDAKYDDKLIAEATKVVMKALNMNGK

PEG4000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K

SOME RESIDUE NUMBERS ARE SIMPLY SKIPPED.

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

beta-lactamase, fluorophore, cephaloridine, HYDROLASE-ANTIBIOTIC complex

KTEMKDDFAKLEEQFDAKLGIFALDTGTNRTVAYRPDERFAFASTIKALTVGVLLQQKSIEDLNQRITYTRDDLVNYNPI
TEKHVDTGMTLKELADASLRYSDNAAQNLILKQIGGPESLKKELRKIGDEVTNPERFEPELNEVNPGETQDTSTARALVT
SLRAFALEDKLPSEKRELLIDWMKRNTTGDALIRAGVPDGWEVADKTGAASYGTRNDIAIIWPPKGDPVVLAVLSSRDKK
DAKYDDKLIAEATKVVMKALNMNGK