Cryptococcus neoformans protein farnesyltransferase in complex with FPT-II and ethylenediamine inhibitor 2

(2R)-3-(cyclohexylamino)-2-hydroxypropane-1-sulfonic acid

[(3,7,11-TRIMETHYL-DODECA-2,6,10-TRIENYLOXYCARBAMOYL)-METHYL]-PHOSPHONIC ACID

Structures of Cryptococcus neoformans Protein Farnesyltransferase Reveal Strategies for Developing Inhibitors That Target Fungal Pathogens.

Cryptococcus neoformans protein farnesyltransferase alpha subunit

Cryptococcus neoformans protein farnesyltransferase beta subunit

MGSSHHHHHHSQLMVTSTYIPMSQRRSWADVKPIMQDDGPNPVVPIMYSEEYKDAMDYFRAIAAKEEKSERALELTEIIV
RMNPAHYTVWQYRFSLLTSLNKSLEDELRLMNEFAVQNLKSYQVWHHRLLLLDRISPQDPVSEIEYIHGSLLPDPKNYHT
WAYLHWLYSHFSTLGRISEAQWGSELDWCNEMLRVDGRNNSAWGWRWYLRVSRPGAETSSRSLQDELIYILKSIHLIPHN
VSAWNYLRGFLKHFSLPLVPILPAILPYTASKLNPDIETVEAFGFPMPSDPLPEDTPLPVPLALEYLADSFIEQNRVDDA
AKVFEKLSSEYDQMRAGYWEFRRRECAEE

MATEFTPSVYSLVSKPLPSNSRPSATLDEQAETEDLISQLFDLTADPNALVSEHGKRYSGLRKQEHTQFLASSFFQLPGK
FVSLDASRPWLVFWTVHSLDLLGVALDQGTKDRVVSTLLHFLSPKGGFGGGPANSQIPHLLPTYASVCSLAIAGNDSSTG
GWKDLAAARQSIYEFFMRCKRPDGGFVVCEGGEVDVRGTYCLLVVATLLDIITPELLHNVDKFVSACQTYEGGFACASFP
FPSVVPSTSAFPTSEPSCRVSMAEAHGGYTSCSLNSHFLLTSVPLPSFPLSIDANAALRWTVLQQGEPIEGGGFRGRTNK
LVDGCYSWWVGGGAPVAEELVRREKSRKVKKSRIEVFEEEKEGDWEDVPPIPPIFNRVALQEFTLVAAQQDPGSTGGLRD
KPGKRPDQYHTCNNLSGLSIAQHKMSHSPSTVSSNRLKFDASKGLPAVKPVAPGGGWKNEDERQNARREIWANALGWIEE
EGGEIIVGGKDNRINTTTPVFNILGLRLKPFINYFYCQEN

PEG4K, CAPSO pH 9.5, lithium sulfate, sodium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 290K

THERE IS NO UNIPORT DATABASE REFERENCE SEQUENCE AVAILABLE AT THE TIME OF THE DEPOSITION. THE SEQUENCES PRESENTED HERE ARE OF FARNESYLTRANSFERASE ALPHA SUBUNIT(RESIDUES 15-349) AND BETA SUBUNIT FROM CRYPTOCOCCUS NEOFORMANS VAR. GRUBII STRAIN H99. THE FIRST 14 RESIDUES IN THE ALPHA SUBUNIT(CHAIN A) REPRESENT EXPRESSION TAG.

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

prenyltransferase, protein farnesyltransferase, TRANSFERASE-TRANSFERASE INHIBITOR complex