Bromide soaked structure of an esterase from the oil-degrading bacterium Oleispira antarctica

Structure and activity of the cold-active and anion-activated carboxyl esterase OLEI01171 from the oil-degrading marine bacterium Oleispira antarctica.

GMSIENLSSNKSFGGWHKQYSHVSNTLNCAMRFAIYLPPQASTGAKVPVLYWLSGLTCSDENFMQKAGAQRLAAELGIAI
VAPDTSPRGEGVADDEGYDLGQGAGFYVNATQAPWNRHYQMYDYVVNELPELIESMFPVSDKRAIAGHSMGGHGALTIAL
RNPERYQSVSAFSPINNPVNCPWGQKAFTAYLGKDTDTWREYDASLLMRAAKQYVPALVDQGEADNFLAEQLKPEVLEAA
ASSNNYPLELRSHEGYDHSYYFIASFIEDHLRFHSNYLNA

0.4M Di-Ammonium citrate, 22% PEG3350, Soaking step: KBr 0.8M, 30s. , pH 4.8, VAPOR DIFFUSION, SITTING DROP, temperature 293K