Crystal structure of the N-terminal and RNase III domains of K. polysporus Dcr1 E224Q mutant

The inside-out mechanism of dicers from budding yeasts.

SNELKVREFYRLHNACVKLKESIKLIYENPLVTDQNVLNLGTAENTIDYTILNTPTLNVAKTLLGNRYSLDLIDLFQSHD
FKDSNTDVDMFIKYPVVYDENLENLAFMHKSFPNMNAHLNDAQKTQLSNERLEFLGDSWLGALVSYIVYTRFPSANEGML
SQMKESIVNNNNLFDWSTKLNFTKRLQGNIATPTRVVKDKMSKRYADCVQAYIGALVIDRFGTEFLDIKEWLEELSEKKL
AKSSQM

200 mM L-proline, 10% PEG3350, 100 mM HEPES buffer pH 7.5, vapor diffusion, temperature 293K

SNELKVREFYRLHNACVKLKESIKLIYENPLVTDQNVLNLGTAENTIDYTILNTPTLNVAKTLLGNRYSLDLIDLFQSHD
FKDSNTDVDMFIKYPVVYDENLENLAFMHKSFPNMNAHLNDAQKTQLSNERLEFLGDSWLGALVSYIVYTRFPSANEGML
SQMKESIVNNNNLFDWSTKLNFTKRLQGNIATPTRVVKDKMSKRYADCVEAYIGALVIDRFGTEFLDIKEWLEELSEKKL
AKSSQM